Chromatic VEP assessment of human macular pigment: Comparison with minimum motion and minimum flicker profiles

To assess the effects of macular pigment optical density (MPOD) on isoluminant stimuli and to quantify MPOD electrophysiologically, MPOD distribution profiles were obtained in normal subjects using minimum motion and minimum flicker photometry. Isoluminance of VEP stimuli was determined using minimum flicker and tritan confusion lines were determined using a minimum distinct border criterion. Onset–offset and reversal VEPs to isoluminant red/green, blue/green, and subject-specific tritan gratings of different diameters were recorded from the same 14 subjects tested psychophysically. VEPs were additionally recorded to annular gratings. Chromatic VEP selectivity was assessed by Fourier analysis and as an index; onset negativity/(onset negativity + onset positivity). Peak MPOD varied between 0.2–0.8. Chromatic onset VEPs to all isoluminant 3-deg fields were predominantly negative. Larger blue/green and tritan stimuli elicited VEPs with additional positive, achromatic components; for 9-deg gratings, peak MPOD showed negative correlation with the power of the VEP fundamental (r = −0.70) and with the selectivity index (r = −0.83). Annular gratings elicited chromatic-specific B/G VEPs but only when isoluminance was determined for the annulus. Chromatic selectivity loss in VEPs to large B/G or Tritan gratings can be used to estimate subject-specific MPOD. An important implication is that isoluminant Tritan stimuli with short-wavelength components must be restricted in size in order to optimize koniocellular selectivity.

[1]  Janus J. Kulikowski,et al.  Integration Times Reveal Mechanisms Responding to Isoluminant Chromatic Gratings: A Two-Centre Visual Evoked Potential Study , 2010 .

[2]  A. Bird,et al.  Comparison of Fundus Autofluorescence and Minimum-Motion Measurements of Macular Pigment Distribution Profiles Derived from Identical Retinal Areas , 2005, Perception.

[3]  B. Hammond,et al.  Spectral Absorbance and Spatial Distribution of Macular Pigment Using Heterochromatic Flicker Photometry , 2005, Optometry and vision science : official publication of the American Academy of Optometry.

[4]  D. Fan,et al.  Macular Pigment Optical Density in a Chinese Sample , 2005, Current eye research.

[5]  A. Morland,et al.  Macular pigments: their characteristics and putative role , 2004, Progress in Retinal and Eye Research.

[6]  Fred W. Fitzke,et al.  Macular pigment density and distribution: comparison of fundus autofluorescence with minimum motion photometry , 2003, Vision Research.

[7]  J. Kulikowski,et al.  Scalp VEPs and intra-cortical responses to chromatic and achromatic stimuli in primates , 2002, Documenta Ophthalmologica.

[8]  J Mellerio,et al.  A portable instrument for measuring macular pigment with central fixation , 2002, Current eye research.

[9]  S A Burns,et al.  Macular pigment density measured by autofluorescence spectrometry: comparison with reflectometry and heterochromatic flicker photometry. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[10]  S. Beatty,et al.  Macular pigment optical density measurement: a novel compact instrument. , 2000, Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians.

[11]  Vittorio Porciatti,et al.  Normative data for onset VEPs to red-green and blue-yellow chromatic contrast , 1999, Clinical Neurophysiology.

[12]  G. Harding,et al.  Morphology of transient VEPs to luminance and chromatic pattern onset and offset , 1999, Vision Research.

[13]  A. G Robson,et al.  Objective specification of tritanopic confusion lines using visual evoked potentials , 1998, Vision Research.

[14]  J. Kulikowski,et al.  Macular pigment and the colour-specificity of visual evoked potentials , 1998, Vision Research.

[15]  A. Weiss,et al.  Development of the spatio-chromatic visual evoked potential (VEP): a longitudinal study , 1998, Vision Research.

[16]  A. Goodchild,et al.  Segregation of receptive field properties in the lateral geniculate nucleus of a New-World monkey, the marmoset Callithrix jacchus. , 1998, Journal of neurophysiology.

[17]  D M Snodderly,et al.  Individual variations in the spatial profile of human macular pigment. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[18]  J. Kulikowski,et al.  Specificity and Selectivity of Chromatic Visual Evoked Potentials , 1996, Vision Research.

[19]  E. Switkes,et al.  Reply to "specificity and selectivity of chromatic visual evoked potentials". , 1996, Vision research.

[20]  Dejan Todorović,et al.  A Gem from the Past: Pleikart Stumpf's (1911) Anticipation of the Aperture Problem, Reichardt Detectors, and Perceived Motion Loss at Equiluminance , 1996 .

[21]  J. Kulikowski,et al.  Variables affecting activation of the blue/yellow pathway using VEPs as an index of specificity , 1996 .

[22]  I J Murray,et al.  Amplitude and phase variations of harmonic components in human achromatic and chromatic visual evoked potentials , 1996, Visual Neuroscience.

[23]  E. Switkes,et al.  Application of the spatiochromatic visual evoked potential to detection of congenital and acquired color-vision deficiencies. , 1993, Journal of the Optical Society of America. A, Optics, image science, and vision.

[24]  D. Flitcroft.  The interactions between chromatic aberration, defocus and stimulus chromaticity: Implications for visual physiology and colorimetry , 1989, Vision Research.

[25]  G B Arden,et al.  Separable evoked retinal and cortical potentials from each major visual pathway: preliminary results. , 1989, The British journal of ophthalmology.

[26]  Bb Lee,et al.  Nonlinear summation of M- and L-cone inputs to phasic retinal ganglion cells of the macaque , 1989, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[27]  Scott Perry,et al.  Color vision testing with a computer graphics system: Preliminary results , 1988, Documenta Ophthalmologica.

[28]  Anthony J. Adams,et al.  Optical density of human macular pigment , 1987, Vision Research.

[29]  DH Hubel,et al.  Psychophysical evidence for separate channels for the perception of form, color, movement, and depth , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[30]  K. Mullen.  The contrast sensitivity of human colour vision to red‐green and blue‐yellow chromatic gratings. , 1985, The Journal of physiology.

[31]  O E Favreau,et al.  Perceived velocity of moving chromatic gratings. , 1984, Journal of the Optical Society of America. A, Optics and image science.

[32]  Carol L. Colby,et al.  The responses of single cells in the lateral geniculate nucleus of the rhesus monkey to color and luminance contrast , 1983, Vision Research.

[33]  G. Plant,et al.  Transient visually evoked potentials to the pattern reversal and onset of sinusoidal gratings. , 1983, Electroencephalography and clinical neurophysiology.

[34]  J. Moreland,et al.  Optimization of a Rayleigh-type equation for the detection of tritanomaly , 1979, Vision Research.

[35]  R. M. Boynton,et al.  Chromatic border perception: The role of red- and green-sensitive cones , 1978, Vision Research.

[36]  J. Kulikowski,et al.  Pattern and movement detection in man and rabbit: Separation and comparison of occipital potentials , 1978, Vision Research.

[37]  V. S. RAMACHANDRAN,et al.  Does colour provide an input to human motion perception? , 1978, Nature.

[38]  D. Regan.  Evoked potentials specific to spatial patterns of luminance and colour. , 1973, Vision research.

[39]  D. Tolhurst,et al.  Psychophysical evidence for sustained and transient detectors in human vision , 1973, The Journal of physiology.

[40]  G. Wyszecki,et al.  Axial chromatic aberration of the human eye. , 1957, Journal of the Optical Society of America.

[41]  Werner Gellermann,et al.  Assessment of the validity of in vivo methods of measuring human macular pigment optical density. , 2006, Optometry and vision science : official publication of the American Academy of Optometry.

[42]  J. Mollon,et al.  THE ARRANGEMENT OF L AND M CONES IN HUMAN AND A PRIMATE RETINA , 2003 .

[43]  J. Kulikowski,et al.  Macular pigment assessment using a colour monitor , 2001 .

[44]  J. Kulikowski,et al.  Red–green and blue–yellow mechanisms are matched in sensitivity for temporal and spatial modulation , 2001, Vision Research.

[45]  J. Kulikowski,et al.  The effect of pattern adaptation on chromatic and achromatic visual evoked potentials , 2001 .

[46]  I. Murray,et al.  Electrophysiological investigation of adult and infant colour vision deficiencies , 1997 .

[47]  J. Kulikowski,et al.  Selective stimulation of colour mechanisms: an empirical perspective. , 1997, Spatial vision.

[48]  J. Kulikowski,et al.  Verification of Human Visual-Evoked Potentials (Veps) Elicited by Gratings Containing Tritanopic Pairs of Hues , 1995 .

[49]  J. Moreland.  Design criteria for a clinical anomaloscope , 1993 .

[50]  J J Kulikowski,et al.  Colour vision: isolating mechanisms in overlapping streams. , 1993, Progress in brain research.

[51]  Janus J. Kulikowski,et al.  On the nature of Visual Evoked Potentials, unit responses and psychophysics. , 1991 .

[52]  D. Carden,et al.  Scalp visual evoked potentials to chromatic and achromatic gratings in macaques with ablated visual area 4. In: Seeing Contour and Colour. , 1989 .

[53]  Ian J. Murray,et al.  Electrophysiological Correlates of Chromatic-Opponent and Achromatic Stimulation in Man , 1989 .

[54]  Ian J. Murray,et al.  Human Visual Evoked-Potentials to Chromatic and Achromatic Gratings , 1987 .

[55]  J. Moreland,et al.  Retinal Distribution of Macular Pigment , 1984 .

[56]  Priv.-Doz. Dr. med. habil. Eberhart Zrenner.  Neurophysiological Aspects of Color Vision in Primates , 1983, Studies of Brain Function.

[57]  P. Cavanagh,et al.  A minimum motion technique for judging equiluminance , 1983 .