Principles of some novel rapid dipstick methods for detection and characterization of verotoxigenic Escherichia coli

Aims: The verotoxigenic Escherichia coli (VTEC) serotype most commonly associated with verotoxin (VT) production is O157:H7, but other serotypes have also been implicated in food‐borne illness. These serotypes exhibit much greater genetic and biochemical diversity than E. coli O157:H7, making screening for all VTEC difficult. Here we describe development and testing of novel multi‐analyte antibody‐based dipstick methods for presumptive detection of VTEC cells and VTs, including non‐O157 serotypes.

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