The use of attenuated total reflectance as tool to monitor the time course of fermentation in wild ferments

Effective fermentation monitoring is a growing need during the production of wine due to the rapid pace of change in the industry, which calls for fast methods providing real-time information in order to reduce stuck and sluggish fermentations and to assure the quality of the product at all stages of the process. During wine fermentation it is important to measure both substrate and product concentrations (e.g. sugars, ethanol) and to evaluate other quality characteristics of the final product, such as the phenolic composition or volatile compounds. However, the analysis of these compounds by traditional methods requires sample preparation, specific analytical equipment and is time-consuming. Therefore real-time monitoring and control of the bioprocesses are necessary for increased productivity, efficiency, and reproducibility. The aim of this study is to evaluate the potential of attenuated total reflectance (ATR) mid infrared (MIR) spectroscopy to monitor wild ferments during wine production. The results obtained showed that it is possible to monitor the time course of fermentation in wild yeast using ATR-MIR spectroscopy. Partial least squares (PLS) regression models allowed to predict the time course of fermentation (standard error of prediction 1.2 days).