Fungus Pichia kudriavzevii XTY1 and heterotrophic nitrifying bacterium Enterobacter asburiae GS2 cannot efficiently transform organic nitrogen via hydroxylamine and nitrite

Heterotrophic nitrification is a process of organic nitrogen degradation completed by the participation of heterotrophic nitrifying microorganisms, which can accelerate the nitrogen transformation process. However, the current research mainly focuses on heterotrophic nitrifying bacteria and their ammonium degradation capacities. And there is little accumulation of research on fungi, the main force of heterotrophic nitrification, and their capacities to transform organic nitrogen. In this study, novel heterotrophic nitrifying fungus (XTY1) and bacterium (GS2) were screened and isolated from upland soil, and the strains were identified and registered through GenBank comparison. After 24 h single nitrogen source tests and 15N labeling tests, we compared and preliminarily determined the heterotrophic nitrification capacities and pathways of the two strains. The results showed that XTY1 and GS2 had different transformation capacities to different nitrogen substrates and could efficiently transform organic nitrogen. However, the transformation capacity of XTY1 to ammonium was much lower than that of GS2. The two strains did not pass through NH2OH and NO2− during the heterotrophic nitrification of organic nitrogen, and mainly generated intracellular nitrogen and low N2O. Other novel organic nitrogen metabolism pathways may be existed, but they remain to be further validated.

[1]  M. Wagner,et al.  Novel Alcaligenes ammonioxydans sp. nov. from wastewater treatment sludge oxidizes ammonia to N2 with a previously unknown pathway. , 2021, Environmental microbiology.

[2]  Xia Huang,et al.  Simultaneous nitrification and aerobic denitrification by a novel isolated Ochrobactrum anthropi HND19. , 2021, Bioresource technology.

[3]  X. Bi,et al.  Enhanced Wet Deposition of Water‐Soluble Organic Nitrogen During the Harvest Season: Influence of Biomass Burning and In‐Cloud Scavenging , 2020, Journal of Geophysical Research: Atmospheres.

[4]  Qingping Wu,et al.  Nitrogen removal characteristics of a versatile heterotrophic nitrifying-aerobic denitrifying bacterium, Pseudomonas bauzanensis DN13-1, isolated from deep-sea sediment. , 2019, Bioresource technology.

[5]  De-ti Xie,et al.  Ammonium transformed into nitrous oxide via nitric oxide by Pseudomonas putida Y-9 under aerobic conditions without hydroxylamine as intermediate. , 2019, Bioresource technology.

[6]  Hu Chen,et al.  Ammonium removal characteristics of an acid-resistant bacterium Acinetobacter sp. JR1 from pharmaceutical wastewater capable of heterotrophic nitrification-aerobic denitrification. , 2019, Bioresource technology.

[7]  P. Bhunia,et al.  Simultaneous removal of nitrogen and phosphorous from domestic wastewater using Bacillus cereus GS-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal. , 2017, Bioresource technology.

[8]  J. Zhao,et al.  Simultaneous removal of carbon and nitrogen by mycelial pellets of a heterotrophic nitrifying fungus-Penicillium sp. L1. , 2017, Journal of bioscience and bioengineering.

[9]  Prepared.  Standard methods for the examination of water and wastewater , 2016 .

[10]  E. Wang,et al.  Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24. , 2015, Bioresource technology.

[11]  Z. Cai,et al.  The substrate is an important factor in controlling the significance of heterotrophic nitrification in acidic forest soils , 2014 .

[12]  Zhouliang Tan,et al.  Impact resistance of different factors on ammonia removal by heterotrophic nitrification-aerobic denitrification bacterium Aeromonas sp. HN-02. , 2014, Bioresource technology.

[13]  김성홍,et al.  Standard Methods for the Examination of Water and Wastewater, 22nd Edition, 2012 , 2012 .

[14]  Yiliang He,et al.  N2O and N2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR. , 2012, Bioresource technology.

[15]  J. Ni,et al.  Ammonium removal by Agrobacterium sp. LAD9 capable of heterotrophic nitrification-aerobic denitrification. , 2012, Journal of bioscience and bioengineering.

[16]  D. Richardson,et al.  Constraining the conditions conducive to dissimilatory nitrate reduction to ammonium in temperate arable soils , 2011 .

[17]  Z. Cai,et al.  Heterotrophic nitrification is the predominant NO3− production mechanism in coniferous but not broad-leaf acid forest soil in subtropical China , 2011, Biology and Fertility of Soils.

[18]  M. Shoda,et al.  Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4. , 2005, Journal of bioscience and bioengineering.

[19]  B. Williams,et al.  Contribution of α-amino N to extractable organic nitrogen (DON) in three soil types from the Scottish uplands , 2005 .

[20]  M. Wood,et al.  Heterotrophic nitrification in an acid forest soil : isolation and characterisation of a nitrifying bacterium , 2001 .

[21]  G. Kowalchuk,et al.  Nitrification in acid soils: micro-organisms and mechanisms , 2001 .

[22]  R. Bol,et al.  Amino acids as a nitrogen source in temperate upland grasslands: the use of dual labelled ((13)C, (15)N) glycine to test for direct uptake by dominant grasses. , 2000, Rapid communications in mass spectrometry : RCM.

[23]  M. Firestone,et al.  The relative importance of autotrophic and heterotrophic nitrification in a conifer forest soil as measured by15N tracer and pool dilution techniques , 1999 .

[24]  F. Pomares,et al.  Evaluation of Chemical Indices of Soil Organic Nitrogen Availability in Calcareous Soils , 1992 .

[25]  J. Kuenen,et al.  Thiosphaera pantotropha gen. nov. sp. nov., a Facultatively Anaerobic, Facultatively Autotrophic Sulphur Bacterium , 1983 .

[26]  D. Frear,et al.  Spectrophotometric Method for Determining Hydroxylamine Reductase Activity in Higher Plants , 1955 .