Characterization of a gastrin releasing peptide from porcine non-antral gastric tissue.

Abstract A heptacosapeptide with potent gastrin releasing activity has been isolated from porcine non-antral gastric and intestinal tissue. The amino acid sequence suggested from a preliminary study on the gastric peptide is: Ala-Pro-Val-Ser-Val-Gly-Gly-Gly-Thr-Val-Leu-Ala-Lys-Met-Tyr-Pro-Arg-Gly-Asn-His-Trp-Ala-Val-Gly-His-Leu-Met-NH2. Striking homology in the C-terminal region is seen with bombesin, accounting for the similar bioactivities of the two peptides. Some structural resemblance with porcine cholecystokinin in the N-terminal region is noted.

[1]  G. Davidson,et al.  VIRUS IN FÆCAL EXTRACTS FROM CHILDREN WITH GASTROENTERITIS , 1974 .

[2]  V. Erspamer,et al.  GASTRIN RELEASE BY BOMBESIN IN THE DOG , 1974, British journal of pharmacology.

[3]  V. Mutt,et al.  Further investigations of intestinal hormonal polypeptides. , 1976, Clinical endocrinology.

[4]  T. Inagami,et al.  Identification of phenylthiohydantoins of amino acids by thin-layer chromatography on a plastic-backed silica-gel plate. , 1972, Analytical biochemistry.

[5]  H. Jörnvall.  Horse liver alcohol dehydrogenase. The primary structure of an N-terminal part of the protein chain of the ethanol-active isoenzyme. , 1970, European journal of biochemistry.

[6]  NormanS. Trac.  BOMBESIN AND THE HUMAN GASTROINTESTINAL TRACT , 1976, The Lancet.

[7]  M. Dhont,et al.  MODULATION OF PITUITARY RESPONSIVENESS TO EXOGENOUS LHRH BY AN OESTROGENIC AND AN ANTI‐OESTROGENIC COMPOUND IN THE NORMAL MALE , 1976, Clinical endocrinology.

[8]  D. Leaback,et al.  Use of Chlorine in the Detection of Compounds on Paper Chromatograms , 1960, Nature.

[9]  C. L. Zimmerman,et al.  Rapid analysis of amino acid phenylthiohydantoins by high-performance liquid chromatography. , 1977, Analytical biochemistry.

[10]  V. Mutt,et al.  Structure of Porcine Cholecystokinin‐Pancreozymin , 1968 .

[11]  S. Waley,et al.  Trypsin-catalysed transpeptidations. , 1954, The Biochemical journal.

[12]  V. Mutt,et al.  On the Biological Activity and Amino Acid Composition of Secretin. , 1961 .

[13]  V. Mutt,et al.  Isolation from porcine-intestinal wall of a vasoactive octacosapeptide related to secretin and to glucagon. , 1972, European journal of biochemistry.

[14]  V. Mutt,et al.  A gastrin releasing peptide from the porcine nonantral gastric tissue. , 1978, Gut.

[15]  J. Polak,et al.  DISTRIBUTION OF A BOMBESIN-LIKE PEPTIDE IN HUMAN GASTROINTESTINAL TRACT , 1976, The Lancet.

[16]  V. Erspamer,et al.  EVIDENCE OF CHOLECYSTOKININ RELEASE BY BOMBESIN IN THE DOG , 1974, British journal of pharmacology.