Rapid tube CAMP test for identification of Streptococcus agalactiae (Lancefield group B)

A rapid CAMP test for the presumptive identification of Streptococcus agalactiae (Lancefield group B) is described. Sheep erythrocytes, sensitized by staphylococcal beta-lysin and suspended in phosphate-buffered saline, were used to determine the lytic capacity of the neutralized supernatant fluids of 4-h broth cultures of streptococci being tested. A total of 96.2% of 130 group B streptococci gave positive CAMP tests, that is, lysis of the sheep erythrocytes after 10 min of exposure of streptococcal supernatants, whereas none of 381 non-group B streptococci tested produced any lysis. The test described provides presumptive identification of group B streptococci within 4 h and eliminates problems of intermediate reactions so that a positive test is indicative only of CAMP factor production.

[1]  R. Cooksey,et al.  Presumptive identification of group A, B, and D streptococci on agar plate media , 1979 .

[2]  H. Wilkinson.  CAMP-disk test for presumptive identification of group B streptococci , 1977, Journal of clinical microbiology.

[3]  C. L. Darling.  Standardization and evaluation of the CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material , 1975, Journal of clinical microbiology.

[4]  G. Wiseman.  FACTORS AFFECTING THE SENSITIZATION OF SHEEP ERYTHROCYTES TO STAPHYLOCOCCAL BETA LYSIN. , 1965, Canadian journal of microbiology.

[5]  G. Wiseman.  SOME CHARACTERISTICS OF THE BETA-HAEMOLYSIN OF STAPHYLOCOCCUS AUREUS. , 1965, The Journal of pathology and bacteriology.

[6]  L. Rantz,et al.  Use of autoclaved extracts of hemolytic streptococci for serological grouping. , 1955, Stanford medical bulletin.

[7]  M. Pulsford.  Some factors influencing the production of CAMP factor by Streptococcus agalactiae. , 1954, The Australian journal of experimental biology and medical science.

[8]  M. Pulsford.  Factors affecting the lysis of erythrocytes treated with staphylococcal beta toxin. , 1954, The Australian journal of experimental biology and medical science.

[9]  E. Munch-Petersen,et al.  On the effect of the interaction of staphylococcal β toxin and group-B streptococcal substance on red blood corpuscles and its use as a test for the identification of Streptococcus agalactæ , 1947 .

[10]  E. Munch-Petersen,et al.  Further notes on a lytic phenomenon shown by group B streptococci. , 1945, The Australian journal of experimental biology and medical science.

[11]  E. Munch-Petersen,et al.  A NOTE ON A LYTIC PHENOMENON SHOWN BY GROUP B STREPTOCOCCI , 1944 .

[12]  John A. Robinson,et al.  Properties of Purified Staphylococcal f 3-Hemolysin , 2022 .