Angiotensinogen and endothelial nitric oxide synthase gene polymorphisms among Hispanic patients with preeclampsia.

OBJECTIVE We sought to establish an association between preeclampsia and the methionine to threonine polymorphism at amino acid residue 235 (Met235Thr) in angiotensinogen in a Hispanic population. We looked for a relationship between this allele and the allele in the endothelial nitric oxide synthase gene (NOS3) that produces the A form (NOS3*A) with respect to preeclampsia. STUDY DESIGN Clinical data were collected from 87 patients with preeclampsia and 53 control subjects. Patients and controls were genotyped for the angiotensinogen polymorphism allele (AGT*T) and the NOS3*A polymorphism. We then compared patients with preeclampsia and control subjects and investigated disease severity within the preeclampsia group as a function of genotype. RESULTS The AGT*T allelic frequencies among patients with preeclampsia and control subjects were 0.72 and 0.70, respectively (P =.84). The blood pressure of patients with an AGT*T allele who also carried a NOS3*A allele was higher at earlier gestational ages (r = -0.052; P =.02). Analysis suggested that the systolic blood pressure differences were due to gestational age effects and the presence of a NOS3*A allele (P <.10). CONCLUSION The AGT*T allele was not associated with the development of preeclampsia. Independently of the presence of an AGT*T allele, the NOS3*A allele was associated with a higher blood pressure at an earlier gestational age.

[1]  E. Steegers,et al.  Polymorphism in the Glutathione S‐transferase P1 Gene and Risk for Preeclampsia , 2000, Obstetrics and gynecology.

[2]  F. Pipkin.  What Is the Place of Genetics in the Pathogenesis of Pre-Eclampsia? , 1999 .

[3]  C. Berg,et al.  Pregnancy-related mortality in Hispanic women in the United States. , 1999, Obstetrics and gynecology.

[4]  R. Ness,et al.  Methylenetetrahydrofolate reductase polymorphism, folate, and susceptibility to preeclampsia. , 1999 .

[5]  J. Connor,et al.  Evidence for a familial pregnancy-induced hypertension locus in the eNOS-gene region. , 1997, American journal of human genetics.

[6]  T. Arinami,et al.  Methylenetetrahydrofolate reductase polymorphism and pre-eclampsia. , 1997, Journal of medical genetics.

[7]  D Paladini,et al.  Factor V Leiden, C>T MTHFR Polymorphism and Genetic Susceptibility to Preeclampsia , 1997, Thrombosis and Haemostasis.

[8]  N. Kalsheker,et al.  Pre‐eclampsia and the angiotensinogen gene , 1995, British journal of obstetrics and gynaecology.

[9]  J. Lalouel,et al.  A molecular variant of angiotensinogen associated with preeclampsia , 1993, Nature Genetics.

[10]  R. Rochat,et al.  Maternal Mortality in the United States: Report From the Maternal Mortality Collaborative , 1988, Obstetrics and gynecology.

[11]  D. Kleinbaum,et al.  Applied Regression Analysis and Other Multivariate Methods , 1978 .

[12]  K. Suzumori,et al.  Is angiotensinogen gene polymorphism associated with hypertension in pregnancy? , 1999, Hypertension in pregnancy.

[13]  S. Brennecke,et al.  Angiotensinogen gene variation in a population case‐control study of preeclampsia/eclampsia in Australians and Chinese , 1997, Electrophoresis.

[14]  B. Sibai,et al.  Urinary dipstick protein: a poor predictor of absent or severe proteinuria. , 1994, American Journal of Obstetrics and Gynecology.