Acetylcholinesterase‐based amperometric sensor

An amperometric sensor has been created using the hydrolysis of acetylthiocholine iodide, which is catalyzed by the acetylcholinesterase enzyme and generates an electroactive product. An electrochemically modified graphite electrode is used, with acetylcholinesterase covalently bonded directly to its surface. The characteristics of the sensor, such as the response time, repeatability, reproducibility, stability, dynamic range, activation energy, and temperature coefficient of the current are evaluated. The optimum working conditions (pH, temperature, and rotation speed) are established.