Furanones as potential anti-bacterial coatings on biomaterials.

A major barrier to the long-term use of medical devices is development of infection. Staphylococcus epidermidis is one of the most common bacterial isolates from these infections with biofilm formation being their main virulence factor. Currently, antibiotics are used as the main form of therapy. However with the emergence of staphylococcal resistance, this form of therapy is fast becoming ineffective. In this study, the ability of a novel furanone antimicrobial compound to inhibit S. epidermidis adhesion and slime production on biomaterials was assessed. Furanones were physically adsorbed to various biomaterials and bacterial load determined using radioactivity. Slime production was assessed using a colorimetric method. Additionally, the effect of the furanone coating on material surface characteristics such as hydrophobicity and surface roughness was also investigated. The results of this study indicated that there was no significant change in the material characteristics after furanone coating. Bacterial load on all furanone-coated materials was significantly reduced (p<0.001) as was slime production (p<0.001). There is a potential for furanone-coated biomaterials to be used to reduce medical device-associated infections.

[1]  P. Sohnle,et al.  In vitro quantitative adherence of bacteria to intravascular catheters. , 1983, The Journal of surgical research.

[2]  G. Peters,et al.  EFFECT OF EXTRACELLULAR SLIME SUBSTANCE FROM STAPHYLOCOCCUS EPIDERMIDIS ON THE HUMAN CELLULAR IMMUNE RESPONSE , 1984, The Lancet.

[3]  C. Edmiston,et al.  In Vitro Evaluation of the Antibacterial Activity of Three Different Central Venous Catheters Against Gram-Positive Bacteria , 2002, European Journal of Clinical Microbiology and Infectious Diseases.

[4]  L. Zhang,et al.  Bromine-, chlorine-, and mixed halogen-substituted 4-methyl-2(5H)-furanones: synthesis and mutagenic effects of halogen and hydroxyl group replacements. , 1997, Chemical research in toxicology.

[5]  R. Darouiche,et al.  Antibiotics and prevention of microbial colonization of catheters , 1995, Antimicrobial agents and chemotherapy.

[6]  J. Keller,et al.  Primary bacterial colonization of implant surfaces. , 1999, The International journal of oral & maxillofacial implants.

[7]  C. Verheyen,et al.  Adherence to a metal, polymer and composite by Staphylococcus aureus and Staphylococcus epidermidis. , 1993, Biomaterials.

[8]  L. Baddour,et al.  Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices , 1985, Journal of clinical microbiology.

[9]  A. Pizzoferrato,et al.  Seven surgical silicones retain Staphylococcus aureus differently in vitro. , 1995, Biomaterials.

[10]  M. Gümüşderelioğlu,et al.  Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials , 2002, Journal of biomaterials science. Polymer edition.

[11]  G. Quinn,et al.  Experimental Design and Data Analysis for Biologists , 2002 .

[12]  M. Davies,et al.  In vitro assessment of bacterial adhesion to Hydromer-coated cerebrospinal fluid shunts. , 1993, Biomaterials.

[13]  J. Costerton,et al.  Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm , 2002, Journal of bacteriology.

[14]  T. Wood,et al.  Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)‐4‐bromo‐5‐(bromomethylene)‐3‐butyl‐2(5H)‐furanone , 2002, Letters in applied microbiology.

[15]  P. Sohnle,et al.  Scanning electron microscopy of bacteria adherent to intravascular catheters , 1984, Journal of clinical microbiology.

[16]  B. Jansen,et al.  Attachment of Staphylococci to Various Synthetic Polymers , 1984 .

[17]  N. Frimodt-Møller,et al.  Attachment of staphylococci to different plastic tubes in vitro. , 1994, Journal of medical microbiology.

[18]  L. Mermel,et al.  Prevention of Central Venous Catheter-Related Bloodstream Infection by Use of an Antiseptic-Impregnated Catheter , 1997, Annals of Internal Medicine.

[19]  S. Kjelleberg,et al.  Eukaryotic interference with homoserine lactone-mediated prokaryotic signalling , 1996, Journal of bacteriology.

[20]  M. R. Brown,et al.  Effect of biofilm culture upon the susceptibility of Staphylococcus epidermidis to tobramycin. , 1992, The Journal of antimicrobial chemotherapy.

[21]  G. Peters,et al.  Interference with granulocyte function by Staphylococcus epidermidis slime , 1986, Infection and immunity.

[22]  S. Kunze,et al.  Use of scanning electron microscopy to investigate the prophylactic efficacy of rifampin-impregnated CSF shunt catheters. , 2000, Journal of medical microbiology.

[23]  M. Ringer,et al.  An attachable silver-impregnated cuff for prevention of infection with central venous catheters: a prospective randomized multicenter trial. , 1988, The American journal of medicine.

[24]  M. Rupp,et al.  Coagulase-negative staphylococci: pathogens associated with medical progress. , 1994, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[25]  A. Crémieux,et al.  Influence of strain, biomaterial, proteins, and oncostatic chemotherapy on Staphylococcus epidermidis adhesion to intravascular catheters in vitro. , 1996, The Journal of laboratory and clinical medicine.

[26]  T. Elliott,et al.  Influence of surface morphology on in vitro bacterial adherence to central venous catheters. , 1994, British journal of anaesthesia.

[27]  G. Peters,et al.  Adherence and growth of coagulase-negative staphylococci on surfaces of intravenous catheters. , 1982, The Journal of infectious diseases.

[28]  G. Pulverer,et al.  Controlled release of antibiotics from biomedical polyurethanes: morphological and structural features. , 1997, Biomaterials.

[29]  R. Darouiche.  Device-associated infections: a macroproblem that starts with microadherence. , 2001, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[30]  É. Voronina,et al.  Synthesis and antimicrobial activity of 4-aroyl-3-hydroxy-2,5-dihydrofuran-2-ones and their derivatives , 2000, Pharmaceutical Chemistry Journal.

[31]  K. Kristinsson.  Adherence of staphylococci to intravascular catheters. , 1989, Journal of medical microbiology.

[32]  H. C. van der Mei,et al.  Initial adhesion and surface growth of Staphylococcus epidermidis and Pseudomonas aeruginosa on biomedical polymers. , 2000, Journal of biomedical materials research.

[33]  D. Schurman,et al.  Quantitation of glycocalyx production in coagulase‐negative Staphylococcus , 1988, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[34]  F. Vandenesch,et al.  Emergence and Spread in French Hospitals of Methicillin-Resistant Staphylococcus aureus with Increasing Susceptibility to Gentamicin and Other Antibiotics , 1999, Journal of Clinical Microbiology.

[35]  S. Kjelleberg,et al.  Inhibitory Effects of Secondary Metabolites from the Red Alga Delisea pulchra on Swarming Motility of Proteus mirabilis , 1996, Applied and environmental microbiology.

[36]  J. Costerton,et al.  Dynamic interactions of biofilms of mucoid Pseudomonas aeruginosa with tobramycin and piperacillin , 1992, Antimicrobial Agents and Chemotherapy.

[37]  A. Husain,et al.  Syntheses and reactions of some new 2-arylidene-4-(biphenyl-4-yl)-but-3-en-4-olides with a study of their biological activity. , 2002, Pharmazie.

[38]  H. C. van der Mei,et al.  In vitro and in vivo antimicrobial activity of covalently coupled quaternary ammonium silane coatings on silicone rubber. , 2002, Biomaterials.

[39]  P. Handley,et al.  The combined effects of plasma and hydrogel coating on adhesion of Staphylococcus epidermidis and Staphylococcus aureus to polyurethane catheters. , 1996, FEMS microbiology letters.

[40]  J. Bellón,et al.  In vitro interaction of bacteria with polypropylene/ePTFE prostheses. , 2001, Biomaterials.

[41]  T. Wood,et al.  Inhibition of biofilm formation and swarming of Escherichia coli by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone. , 2001, Environmental microbiology.

[42]  I. Raad,et al.  Intravascular-catheter-related infections , 1998, The Lancet.

[43]  A. Seldes,et al.  5H-furan-2-ones from fungal cultures of Aporpium caryae. , 2003, Phytochemistry.

[44]  A. Janecka,et al.  New stereocontrolled synthesis and biological evaluation of 5-(1'-hydroxyalkyl)-3-methylidenetetrahydro-2-furanones as potential cytotoxic agents. , 2002, Journal of medicinal chemistry.

[45]  Y. An,et al.  Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. , 1998, Journal of biomedical materials research.