论文信息 - The expression of the bstVIM gene from Bacillus stearothermophilus V is restricted to vegetative cell growth.

The expression of the bstVIM gene from Bacillus stearothermophilus V is restricted to vegetative cell growth.

The activity of BstVI DNA methyltransferase was monitored during the sporulative cycle of Bacillus stearothermophilus V. Significant methylase activity was found only in bacteria growing vegetatively. This was confirmed by Northern hybridization, which indicated that the bstVIM gene was not transcribed in cells undergoing sporulation. Supporting evidence came from experiments which demonstrated that the RNA polymerase holoenzyme from these cells did not recognize the promoter elements upstream of the bstVIM gene.

C. Saavedra | C. Vásquez | C. Padilla | E. González

[1] C. Vásquez,et al. Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system. , 1993, Gene.

[2] J. Errington,et al. Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis. , 1993, Microbiological reviews.

[3] C. Saavedra,et al. Cloning the BstVI restriction-modification system in Escherichia coli. , 1991, Gene.

[4] C. Vásquez. Isolation and partial characterization of BstVI, a thermostable isoschizomer of XhoI. , 1985, Biochemistry International.

[5] R. Losick,et al. Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene , 1983, Nature.

[6] R. Losick,et al. Cascades of sigma factors , 1981, Cell.

[7] P. Piggot,et al. Genetic aspects of bacterial endospore formation. , 1976, Bacteriological reviews.

[8] M. Chamberlin,et al. Structure and function of bacterial sigma factors. , 1988, Annual review of biochemistry.

[9] R. Losick,et al. Genetics of endospore formation in Bacillus subtilis. , 1986, Annual review of genetics.