Stand-Alone Video-Based Animal Tracking System for Noiseless Application

Abstract The acoustic environment is an important variable in studies with animal behavior and psychology. Noise represents an important factor in laboratory settings. Noise of moderate intensity is produced by air conditioning devices and experimental equipment. Traditional automated video tracking systems require one personal computer per monitoring platform, which are noisy and inconvenient for large laboratory applications. This paper presents a stand-alone system for this function (noise-control environment/noiseless application). A novel system combination of FPGA and MCU simultaneously and automatically perform tracking and saving acquired data to an SD card. A fast and efficient tracking algorithm is designed and implemented. Detection via the designed algorithm uses the Y (luminance component), Cb (blue-difference chrominance component) and Cr (red-difference chrominance component) value of a colored marker affixed to the target to define the tracked position and allows multi-object tracking against complex backgrounds. The tracking result is updated every 30 frames/second and only the locomotive data are stored. The proposed system can track animal's locomotion totally noiseless operating.

[1]  HARALD KREBS,et al.  Effects of Stressful Noise on Eating and Non-eating Behavior in Rats , 1996, Appetite.

[2]  Michael Macht,et al.  Scanning Behavior of Rats During Eating Under Stressful Noise , 1997, Physiology & Behavior.

[3]  J. Crabbe,et al.  Genetics of mouse behavior: interactions with laboratory environment. , 1999, Science.

[4]  S. Fowler,et al.  A force-plate actometer for quantitating rodent behaviors: illustrative data on locomotion, rotation, spatial patterning, stereotypies, and tremor , 2001, Journal of Neuroscience Methods.

[5]  Leonie de Visser,et al.  Automated home cage observations as a tool to measure the effects of wheel running on cage floor locomotion , 2005, Behavioural Brain Research.

[6]  Ivan Krekule,et al.  An automatic 3D tracking system with a PC and a single TV camera , 1999, Journal of Neuroscience Methods.

[7]  T. Hirai,et al.  Effects of perinatal stress on the anxiety-related behavior of the adolescence mouse , 2006, International Journal of Developmental Neuroscience.

[8]  Helmut Prior Effects of the acoustic environment on learning in rats , 2006, Physiology & Behavior.

[9]  A. J Spink,et al.  The EthoVision video tracking system—A tool for behavioral phenotyping of transgenic mice , 2001, Physiology & Behavior.

[10]  Ming-Shing Young,et al.  Integrated digital image and accelerometer measurements of rat locomotor and vibratory behaviour , 2007, Journal of Neuroscience Methods.

[11]  Kathyrne Mueller,et al.  Time course of amphetamine-induced locomotor stereotypy in an open field , 2004, Psychopharmacology.

[12]  M. Grossmann,et al.  A simple computer based system to analyze Morris water maze trials on-line , 1996, Journal of Neuroscience Methods.

[13]  N S Zefirov,et al.  Versatile computerized system for tracking and analysis of water maze tests , 2001, Behavior research methods, instruments, & computers : a journal of the Psychonomic Society, Inc.

[14]  L P Noldus,et al.  EthoVision: A versatile video tracking system for automation of behavioral experiments , 2001, Behavior research methods, instruments, & computers : a journal of the Psychonomic Society, Inc.

[15]  Shuping Ye,et al.  A simple video position-digitizer for studying animal movement patterns , 1991, Journal of Neuroscience Methods.

[16]  C. W. Young,et al.  A combined system for measuring animal motion activities , 2000, Journal of Neuroscience Methods.

[17]  Helmut Prior,et al.  Effects of predictable and unpredictable intermittent noise on spatial learning in rats , 2002, Behavioural Brain Research.

[18]  Valter Tucci,et al.  Gene-environment interactions differentially affect mouse strain behavioral parameters , 2006, Mammalian Genome.

[19]  T. Ono,et al.  A combined electrophysiological and video data acquisition system using a single computer , 1999, Journal of Neuroscience Methods.

[20]  D. Cross,et al.  Another look at amphetamine-induced stereotyped locomotor activity in rats using a new statistic to measure locomotor stereotypy , 2004, Psychopharmacology.

[21]  Charles F. Babbs,et al.  A novel open field activity detector to determine spatial and temporal movement of laboratory animals after injury and disease , 2006, Journal of Neuroscience Methods.

[22]  Hans-Peter Lipp,et al.  Extended analysis of path data from mutant mice using the public domain software Wintrack , 2001, Physiology & Behavior.

[23]  C. W. Young,et al.  A new ultrasonic method for measuring minute motion activities on rats , 1996, Journal of Neuroscience Methods.

[24]  F. K. Lam,et al.  A novel system for simultaneous monitoring of locomotor and sound activities in animals , 2000, Journal of Neuroscience Methods.

[25]  M. S. Young,et al.  The development of a low cost animal behavior measurement system , 1999, Proceedings of the First Joint BMES/EMBS Conference. 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Annual Fall Meeting of the Biomedical Engineering Society (Cat. N.

[26]  H. Würbel,et al.  Behavioral phenotyping enhanced – beyond (environmental) standardization , 2002, Genes, brain, and behavior.

[27]  Kohji Tokumo,et al.  Effects of perinatal stress on the emotion-related behavior of the adolescent mouse. , 2006, Biological & pharmaceutical bulletin.

[28]  Kathyrne Mueller,et al.  Locomotor Stereotypy Produced by Dexbenzetimide and Scopolamine Is Reduced by SKF 83566, Not Sulpiride , 1998, Pharmacology Biochemistry and Behavior.

[29]  Michael Macht,et al.  Effect of stress on feeding behavior in rats: individual differences , 2001 .