Formation of neuromuscular junctions in embryonic cultures.

Formation of neuromuscular junctions in embryonic cultures has been reviewed. Anatomical and electrophysiological evidence has been presented which demonstrates the formation de novo of synaptic contacts between nerve and muscle in culture; in (1) organ cultures of spinal cord explanted intact with attached myotomes, (2) organ cultures of in vitro coupled explants of spinal cord and skeletal muscle fragments, and (3) monolayer cultures of dissociated myoblasts confronted either with spinal cord fragments or cells. Innervation across species lines has also been demonstrated in explants of spinal cord and skeletal muscle from different species These cultures should provide a useful system for analysis of the mechanisms underlying the trophic and other interactions during neuromuscular development.

[1]  Y. Shimada,et al.  Innervation and acetylcholine sensitivity of skeletal muscle cells differentiatedin vitro from chick embryo , 1971, Journal of cellular physiology.

[2]  Y. Shimada,et al.  Innervation of skeletal muscle cells differentiated in vitro from chick embryo. , 1971, Brain research.

[3]  Y. Shimada,et al.  Acetylcholine sensitivity of skeletal muscle cells differentiated in vitro from chick embryo. , 1971, Brain research.

[4]  G. Fischbach,et al.  Synaptic Potentials Recorded in Cell Cultures of Nerve and Muscle , 1970, Science.

[5]  D. Fambrough.  Acetylcholine Sensitivity of Muscle Fiber Membranes: Mechanism of Regulation by Motoneurons , 1970, Science.

[6]  S. Crain.  Bioelectric interactions between cultured fetal rodent spinal cord and skeletal muscle after innervation in vitro. , 1970, The Journal of experimental zoology.

[7]  Harris Aj,et al.  Acetylcholine Sensitivity and Distribution on Mouse Neuroblastoma Cells , 1970 .

[8]  D. Fischman,et al.  THE DEVELOPMENT OF NERVE-MUSCLE JUNCTIONS IN MONOLAYER CULTURES OF EMBRYONIC SPINAL CORD AND SKELETAL MUSCLE CELLS , 1969, The Journal of cell biology.

[9]  A. Kelly,et al.  THE FINE STRUCTURE OF MOTOR ENDPLATE MORPHOGENESIS , 1969, The Journal of cell biology.

[10]  D. Fischman,et al.  Formation of neuromuscular junctions in embryonic cell cultures. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[11]  L. Guth.  "Trophic" influences of nerve on muscle. , 1968, Physiological reviews.

[12]  D. W. James,et al.  De novo Formation of Neuro-muscular Junctions in Tissue Culture , 1968, Nature.

[13]  Y. Shimada.  Suppression of myogenesis by heterotypic and heterospecific cells in monolayer cultures. , 1968, Experimental cell research.

[14]  H. Hirano.  A histochemical study of the cholinesterase activity in the neuromuscular junction in developing chick skeletal muscles. , 1967, Archivum histologicum Japonicum = Nihon soshikigaku kiroku.

[15]  M. Corner.  RHYTHMICITY IN THE EARLY SWIMMING OF ANURAN LARVAE. , 1964, Journal of embryology and experimental morphology.

[16]  M. Karnovsky.  THE LOCALIZATION OF CHOLINESTERASE ACTIVITY IN RAT CARDIAC MUSCLE BY ELECTRON MICROSCOPY , 1964, The Journal of cell biology.

[17]  M. Corner,et al.  Localization of capacities for functional development in the neural plate of Xenopus laevis , 1964, The Journal of comparative neurology.

[18]  M. Karnovsky,et al.  A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES , 1964, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[19]  B. Katz,et al.  The development of acetylcholine sensitivity in nerve‐free segments of skeletal muscle , 1964, The Journal of physiology.

[20]  R. Miledi.  Formation of Extra Nerve-muscle Junctions in Innervated Muscle , 1963, Nature.

[21]  D. Ottoson,et al.  Observations on the functional development of the neuromuscular apparatus in fetal sheep. , 1963, Experimental neurology.

[22]  R. Miledi,et al.  A study of foetal and new‐born rat muscle fibres , 1962, The Journal of physiology.

[23]  R. Miledi.  Junctional and extra‐junctional acetylcholine receptors in skeletal muscle fibres , 1960, The Journal of physiology.

[24]  J. Eccles,et al.  Interactions between motoneurones and muscles in respect of the characteristic speeds of their responses , 1960, The Journal of physiology.

[25]  C. Li,et al.  Some properties of cultured chick skeletal muscle with particular reference to fibrillation potential. , 1959, Journal of Cellular and Comparative Physiology.

[26]  B. L. Ginsborg,et al.  The electrical properties of the slow muscle fibre membrane , 1956, The Journal of physiology.

[27]  B. Katz,et al.  On the localization of acetylcholine receptors , 1955, The Journal of physiology.

[28]  S. W. Kuffler,et al.  Small‐nerve junctional potentials. The distribution of small motor nerves to frog skeletal muscle, and the membrane characteristics of the fibres they innervate * , 1953, The Journal of physiology.

[29]  C. Kupfer,et al.  A histochemical study of cholinesterase during formation of the motor end plate of the albino rat. , 1951, The Journal of experimental zoology.

[30]  G B KOELLE,et al.  A Histochemical Method for Localizing Cholinesterase Activity.* , 1949, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[31]  J. Szepsenwol.  Electrical excitability and spontaneous activity in explants of skeletal and heart muscle of chick embryos , 1947, The Anatomical record.

[32]  J. Szepsenwol,et al.  A comparison of growth, differentiation, activity and action currents of heart and skeletal muscle in tissue culture , 1946, The Anatomical record.

[33]  P. Weiss,et al.  Further experimental evidence against “neurotropism” in nerve regeneration , 1944 .

[34]  W. F. Windle,et al.  Development of reflex mechanisms in the spinal cord of albino rat embryos. Correlations between structure and function, and comparisons with the cat and the chick , 1936 .

[35]  W. F. Windle,et al.  The Origin and Early Development of Somatic Behavior in the Albino Rat , 1935, Physiological Zoology.

[36]  W. F. Windle.  Correlation between the development of local reflexes and reflex arcs in the spinal cord of cat embryos , 1934 .

[37]  A. Gonźlez,et al.  The prenatal development of behavior in the albino rat , 1932 .

[38]  W. F. Windle,et al.  The neurofibrillar structure of the spinal cord of cat embryos correlated with the appearance of early somatic movements , 1931 .

[39]  R. G. Harrison,et al.  Observations on the living developing nerve fiber , 1906 .

[40]  S. Crain,et al.  Regeneration and Innervation, de Novo, of Adult Skeletal Muscle in Vitro , 1970 .

[41]  S. Crain,et al.  Neuromuscular transmission in cultures of adult human and rodent skeletal muscle after innervation in vitro by fetal rodent spinal cord. , 1970, Journal of neurobiology.

[42]  J. Nakai.  The development of neuromuscular junctions in cultures of chick embryo tissues. , 1969, The Journal of experimental zoology.

[43]  G. Gabella,et al.  The development of neuro-muscular correlations, in vertebrates. , 1967, Archives de biologie.

[44]  S M Crain,et al.  Development of "organotypic" bioelectric activities in central nervous tissues during maturation in culture. , 1966, International review of neurobiology.

[45]  S. Zacks.  The motor endplate , 1965 .