Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches

A Bacillus species that produces 2,3-butanediol (2,3-BD), termed BRC1, was newly isolated, and a 2,3-BD dehydrogenase (Bdh) from this species was identified and characterized at the molecular and biochemical level. Sequence analysis revealed that Bdh is homologous to D-2,3-BD dehydrogenases. An analysis of the enzymatic properties of Bdh overexpressed in Escherichia coli confirmed the molecular results, showing preferred activity toward D-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Overexpression of bdh under the control of a xylose-inducible promoter resulted in increased enzyme activity and enhanced 2,3-BD production in Bacillus sp. BRC1. Additionally, a hydrolysate of cellulosic material, (empty palm fruit bunches), was successfully used for the enhanced production of 2,3-BD in the recombinant Bacillus strain.

[1]  Cuiqing Ma,et al.  Production of 2,3-butanediol from corncob molasses, a waste by-product in xylitol production , 2010, Applied Microbiology and Biotechnology.

[2]  He Huang,et al.  Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactivation of acetaldehyde dehydrogenase gene , 2010, Applied Microbiology and Biotechnology.

[3]  Cuiqing Ma,et al.  Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM , 2009, Applied Microbiology and Biotechnology.

[4]  J. Hamann,et al.  Enhanced 2,3-butanediol production in fed-batch cultures of free and immobilized Bacillus licheniformis DSM 8785 , 2013, Applied Microbiology and Biotechnology.

[5]  Sha Li,et al.  Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol. , 2010, Bioresource technology.

[6]  Shangtian Yang,et al.  Production of 2,3‐butanediol from glucose by GRAS microorganism Bacillus amyloliquefaciens , 2011, Journal of basic microbiology.

[7]  Jia Luo,et al.  Production of 2,3-butanediol from cellulose and Jatropha hulls after ionic liquid pretreatment and dilute-acid hydrolysis , 2013, AMB Express.

[8]  D. Seung,et al.  Isolation and characterization of a new mucoid-free Klebsiella pneumoniae strain for 2,3-butanediol production , 2012 .

[9]  G. Guo,et al.  Method of 2,3-butanediol production from glycerol and acid-pretreated rice straw hydrolysate by newly isolated strains: pre-evaluation as an integrated biorefinery process. , 2013, Bioresource technology.

[10]  P. Ouyang,et al.  Microbial 2,3-butanediol production: a state-of-the-art review. , 2011, Biotechnology advances.

[11]  Won-Kyung Hong,et al.  Efficient Production of Ethanol from Empty Palm Fruit Bunch Fibers by Fed-Batch Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae , 2013, Applied Biochemistry and Biotechnology.

[12]  Jibin Sun,et al.  Novel (2R,3R)-2,3-Butanediol Dehydrogenase from Potential Industrial Strain Paenibacillus polymyxa ATCC 12321 , 2011, Applied and Environmental Microbiology.

[13]  P. Petrova,et al.  High production of 2,3-butanediol from glycerol by Klebsiella pneumoniae G31 , 2009, Applied Microbiology and Biotechnology.

[14]  Shangtian Yang,et al.  Improved Production of 2,3-Butanediol in Bacillus amyloliquefaciens by Over-Expression of Glyceraldehyde-3-Phosphate Dehydrogenase and 2,3-butanediol Dehydrogenase , 2013, PloS one.

[15]  Z. Xiu,et al.  Microbial production of 2,3-butanediol from Jerusalem artichoke tubers by Klebsiella pneumoniae , 2009, Applied Microbiology and Biotechnology.

[16]  Cuiqing Ma,et al.  A newly isolated Bacillus licheniformis strain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical , 2013, Biotechnology for Biofuels.

[17]  Zhen Fang,et al.  Production of 2,3-butanediol from acid hydrolysates of Jatropha hulls with Klebsiella oxytoca. , 2012, Bioresource technology.

[18]  W. Grajek,et al.  Biotechnological production of 2,3-butanediol--current state and prospects. , 2009, Biotechnology advances.

[19]  Young-Rok Kim,et al.  Removal of pathogenic factors from 2,3-butanediol-producing Klebsiella species by inactivating virulence-related wabG gene , 2012, Applied Microbiology and Biotechnology.

[20]  Jang-Min Park,et al.  Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae , 2013, Biotechnology and Bioprocess Engineering.

[21]  M. Syu.  Biological production of 2,3-butanediol , 2001, Applied Microbiology and Biotechnology.

[22]  G. T. Tsao,et al.  Production of 2,3- butanediol from pretreated corn cob byKlebsiella oxytoca in the presence of fungal cellulase , 1997, Applied biochemistry and biotechnology.

[23]  Cuiqing Ma,et al.  Efficient 2,3-Butanediol Production from Cassava Powder by a Crop-Biomass-Utilizer, Enterobacter cloacae subsp. dissolvens SDM , 2012, PloS one.

[24]  Z. Xiu,et al.  A novel strategy for integrated utilization of Jerusalem artichoke stalk and tuber for production of 2,3-butanediol by Klebsiella pneumoniae. , 2010, Bioresource technology.