Cyclic AMP‐dependent protein kinases from Balb 3T3 cells and other 3T3 derived lines

Cyclic AMP‐dependent protein kinase and 3H‐cAMP‐binding activities were determined in normal Balb 3T3 cells and compared with the same preparations from SV40, chemical, and spontaneous transformants of 3T3 cells. The cytosolic protein kinase activities and protein kinase activity ratios were similar in all cell lines, although when the normal 3T3 cytosol was prepared by homogenization it contained less 3H‐cAMP binding activity than the transformed 3T3 cytosols. The Triton X‐100 treated particulate fractions from the normal and transformed 3T3 cells contained similar protein kinase and binding activities.

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