Based on the sequence alignment of the 3-1E gene of Eimeria acervulina(E.acervulina)US strain,two pairs of primers were designed and synthesized.Using the total RNA of merozoites of E.acervulina Baoding strain isolated from Hebei province of China as template,a partial segment of 3-1E gene was amplified by RT-PCR.The gene fragment 689 bp in length was cloned into pGM-T Easy vector,and the recombinant plasmid was identified by PCR、restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the 3-1E gene of the E.acervulina Baoding strain similar to the US strain and QH strain were 99.4% and 99.6%,respectively.