Lectin binding patterns in hyperplastic and metaplastic bullock prostate tissues after diethyistilbestrol administration

Hyperplasia and squamous metaplasia of the prostatic epithelium are conditions induced by oestrogens. Diethylstilbestrol (DES) has been banned from cattle used for beef production because of the health risks. The potential use of molecular markers for the detection of illegal oestrogen administration was evaluated by taking samples of prostatic tissue from control bullocks, bullocks which had been treated with oestrogens, and bullocks sacrificed 21 and 90 days after a single dose of DES. The expression of the glycoconjugates was examined by lectinhistochemistry and the lectin binding pattern was characterised in epithelium and connective tissue. In the animals sacrificed after 21 days there was an increase in the binding of one lectin (JAc) and there was an increase in the binding of one of the other lectins (DBA) in the animals sacrificed after 90 days. An increase in SWGA lectin staining was observed in the bullocks that had probably been treated with oestrogen and in the animals sacrificed 90 days after the inoculation with DES. There were also differences between the binding of SWGA in the control bullocks and the other groups.

[1]  J. Fioramonti,et al.  Effects of treatment with smectite on gastric and intestinal glycoproteins in the rat: A histochemical study , 1987, The Histochemical Journal.

[2]  A. Tsukise,et al.  Secretory glycoconjugates in the epithelium of the goat prostate , 1987, The Histochemical Journal.

[3]  M. Arenas,et al.  A lectin histochemistry comparative study in human normal prostate, benign prostatic hyperplasia, and prostatic carcinoma , 1999, Glycoconjugate Journal.

[4]  G. Aumüller.  Morphologic and regulatory aspects of prostatic function , 2004, Anatomy and Embryology.

[5]  G. Aumüller,et al.  Intracellular localization of prostatic binding protein (PBP) in rat prostate by light and electron microscopic immunocytochemistry , 2004, Histochemistry.

[6]  E. Gimeno,et al.  Lectin histochemical study of lipopigments present in the cerebellum of Solanum fastigiatum var. fastigiatum intoxicated cattle. , 2002, Journal of veterinary medicine. A, Physiology, pathology, clinical medicine.

[7]  E. Portiansky,et al.  Intermediate filament protein expression and sugar moieties in normal canine placenta. , 2000, Histology and histopathology.

[8]  Y. Martín.  Determination of three anabolic compounds in calf urine by liquid chromatography with photodiode-array detection. , 2000, The Analyst.

[9]  E. Portiansky,et al.  Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate , 1999 .

[10]  S. Ho,et al.  Comparative study of glycoconjugates of the rat prostatic lobes by lectin histochemistry , 1999, The Prostate.

[11]  J. B. Morgan,et al.  Anabolic implant effects on visceral organ mass, chemical body composition, and estimated energetic efficiency in cloned (genetically identical) beef steers. , 1997, Journal of animal science.

[12]  F. Ramaekers,et al.  Immunohistochemical method for detecting lesions in the prostate gland of bulls treated with diethylstilbestrol-dipropionate , 1996, Veterinary Record.

[13]  J. Papadimitriou,et al.  Aberrant pattern of lectin binding in low and high grade prostatic intraepithelial neoplasia , 1995, Cancer.

[14]  H. Gabius,et al.  Androgen status and expression of glycoconjugates and lectins in the epithelial cells of the mouse ventral prostate. A glycohistochemical approach. , 1994, Histology and histopathology.

[15]  W A Wells,et al.  Equipment, standardization, and applications of image processing. , 1993, American journal of clinical pathology.

[16]  S. Wakui,et al.  Lectin histochemical study of the prostate gland of the rhesus monkey (Macaca mulatta). , 1992, Journal of anatomy.

[17]  Y. Wong,et al.  Glycoconjugates of the lateral prostate of the guinea pig: A lectin histochemical study , 1991, The Prostate.

[18]  S. Epstein.  The Chemical Jungle: Today's Beef Industry , 1990, International journal of health services : planning, administration, evaluation.

[19]  I. Leav,et al.  Differential lectin staining of central and peripheral zones of the prostate and alterations in dysplasia. , 1988, American journal of clinical pathology.

[20]  J. Suominen,et al.  Distribution of glycoconjugates in normal rat ventral prostate and their use as markers of androgen‐controlled secretory function in culture , 1986, The Prostate.

[21]  J. Alroy,et al.  Histochemical studies of epithelial cell glycoconjugates in atrophic, metaplastic, hyperplastic, and neoplastic canine prostate. , 1984, Laboratory investigation; a journal of technical methods and pathology.

[22]  Spicer Ss,et al.  Identification of cell surface constituents. , 1982 .

[23]  S. Spicer,et al.  Identification of cell surface constituents. , 1982, Laboratory investigation; a journal of technical methods and pathology.

[24]  G. Aumüller,et al.  Age‐dependent changes in the carbohydrate pattern of human prostatic epithelium as determined by peroxidase‐labeled lectins , 1982, The Prostate.

[25]  I. Goldstein,et al.  The lectins: carbohydrate-binding proteins of plants and animals. , 1978, Advances in carbohydrate chemistry and biochemistry.

[26]  R. Stephany,et al.  Methods for controlling the application of anabolics in farm animals. , 1976, Environmental quality and safety. Supplement.

[27]  R. Kroes,et al.  Evaluation of the "prostate test" in checking the administration of oestrogens in the calf. , 2010, Zentralblatt fur Veterinarmedizin. Reihe A.