Real‐time two‐photon confocal microscopy using a femtosecond, amplified Ti:sapphire system

The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real‐time two‐photon imaging. It is shown that the sectioning inherent to two‐photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high‐power, low‐repetition‐rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.

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