Carbohydrate components recognized by the cone‐specific monoclonal antibody CSA‐1 and by peanut agglutinin are associated with red and green‐sensitive cone photoreceptors

Previous investigations have demonstrated that the monoclonal antibody CSA‐1 and peanut agglutinin label specifically cone photoreceptor cells. In the present study, we compared the binding of CSA‐1 and peanut agglutinin to that of the monoclonal antibodies COS‐1 and OS‐2, which have been shown to recognize the red/green‐ and blue‐sensitive cone visual pigments, respectively. Using lectin and immunocytochemistry on serial semithin sections of the pig retina, we have demonstrated in the present study that both CSA‐1 and peanut agglutinin label specifically the red‐, and green‐sensitive, but not the blue‐sensitive cone cell outer segments. Peanut agglutinin does bind, however, to the cone matrix sheaths associated with all three types of cones. These observations support the idea that red‐, and green‐sensitive cone cells share some common molecular epitopes and may represent a differentiation line of cones, considerably different from that of blue‐sensitive cones.

[1]  Á. Szél,et al.  Four photoreceptor types in the ground squirrel retina as evidenced by immunocytochemistry , 1988, Vision Research.

[2]  Á. Szél,et al.  Identification of the blue‐sensitive cones in the mammalian retina by anti‐visual pigment antibody , 1988, The Journal of comparative neurology.

[3]  G. Hageman,et al.  Ultrastructural visualization of primate cone photoreceptor matrix sheaths , 1988, The Journal of comparative neurology.

[4]  G. Hageman,et al.  Characterization of molecules bound by the cone photoreceptor-specific monoclonal antibody CSA-1. , 1988, Investigative ophthalmology & visual science.

[5]  N. Ohba,et al.  Specialization of the interphotoreceptor matrices around cone and rod photoreceptor cells in the monkey retina, as revealed by lectin cytochemistry. , 1987, Experimental eye research.

[6]  Á. Szél,et al.  Monoclonal antibody-recognizing cone visual pigment. , 1986, Experimental eye research.

[7]  Á. Szél,et al.  Immunocytochemical discrimination of visual pigments in the retinal photoreceptors of the nocturnal gecko Teratoscincus scincus. , 1986, Experimental eye research.

[8]  G. Hageman,et al.  Biochemical characterization of the major peanut‐agglutinin‐binding glycoproteins in vertebrate retinae , 1986, The Journal of comparative neurology.

[9]  V. Lemmon.  A monoclonal antibody that binds to cones. , 1986, Investigative ophthalmology & visual science.

[10]  J. Nathans,et al.  Molecular genetics of human color vision: the genes encoding blue, green, and red pigments. , 1986, Science.

[11]  G. Hageman,et al.  Interphotoreceptor matrix domains ensheath vertebrate cone photoreceptor cells. , 1986, Investigative ophthalmology & visual science.

[12]  J. Blanks,et al.  Specific binding of peanut lectin to a class of retinal photoreceptor cells. A species comparison. , 1984, Investigative ophthalmology & visual science.

[13]  S. Schein,et al.  Staining of blue-sensitive cones of the macaque retina by a fluorescent dye. , 1981, Science.

[14]  R. Marc,et al.  Chromatic organization of primate cones. , 1977, Science.

[15]  I. W. Mclean,et al.  PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[16]  P. Röhlich.  The interphotoreceptor matrix: electron microscopic and histochemical observations on the vertebrate retina. , 1970, Experimental eye research.

[17]  B. Rosario,et al.  A SIMPLE METHOD FOR REMOVING THE RESIN FROM EPOXY-EMBEDDED TISSUE , 1961, The Journal of biophysical and biochemical cytology.

[18]  Á. Szél,et al.  Four cone types characterized by anti-visual pigment antibodies in the pigeon retina. , 1989, Investigative ophthalmology & visual science.

[19]  E. García-Olivares,et al.  Presence of Langerhans cells in the cornea of Klebsiella keratoconjunctivitis mice. , 1988, Investigative ophthalmology & visual science.

[20]  J. Nathans,et al.  Molecular biology of visual pigments. , 1987, Annual review of neuroscience.

[21]  P K Ahnelt,et al.  Identification of a subtype of cone photoreceptor, likely to be blue sensitive, in the human retina , 1987, The Journal of comparative neurology.

[22]  G. Hageman,et al.  Enzymatic characterization of peanut agglutinin-binding components in the retinal interphotoreceptor matrix. , 1987, Experimental eye research.

[23]  Á. Szél,et al.  Localization of visual pigment antigens to photoreceptor cells with different oil droplets in the chicken retina. , 1985, Acta biologica Hungarica.

[24]  T. Muramatsu,et al.  Localization of fluorescence-labeled lectin binding sites on photoreceptor cells of the monkey retina. , 1983, Experimental eye research.

[25]  R. Marc.  Chromatic Organization of the Retina , 1982 .