Immunological effects of amplitude-modulated radio frequency radiation: B lymphocyte capping.

B lymphocytes collected from normal ICR Swiss mouse spleens were exposed in vitro in a Crawford cell to 147-MHz radiofrequency (RF) radiation, amplitude modulated by a 9-, 16-, or 60-Hz sine wave. The power densities ranged between 0.11 and 48 mW/cm2. The irradiated samples and the controls were maintained at 37 degrees C or 42 degrees C, with temperature variations less than 0.1 degrees C. Immediately after a 30-minute exposure, the distribution of antigen-antibody (Ag-Ab) complexes on the cell surface was evaluated at 37 degrees C by immunofluorescence. Under normal conditions (37 degrees C, no RF), Ag-Ab complexes are regrouped into a polar cap by an energy-dependent process. Our results demonstrate that the irradiated cells and the nonirradiated controls capped Ag-Ab complexes equally well after exposure at 37 degrees C. Capping was equally inhibited at 42 degrees C in both the controls and irradiated cells. No statistically significant differences in capping were observed between the RF-exposed and control samples at any of the modulation frequencies and power densities employed as long as both preparations were maintained at the same temperature.

[1]  J P Changeux,et al.  On the cooperativity of biological membranes. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[2]  A. Böyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[3]  G. Schwarz.  Cooperative binding to linear biopolymers. 1. Fundamental static and dynamic properties. , 1970, European journal of biochemistry.

[4]  Myron L. Crawford,et al.  Generation of Standard EM Fields Using TEM Transmission Cells , 1974 .

[5]  W. R. Adey,et al.  EFFECTS OF MODULATED VHF FIELDS ON THE CENTRAL NERVOUS SYSTEM * , 1975, Annals of the New York Academy of Sciences.

[6]  POSSIBLE PHYSICAL SUBSTRATES FOR THE INTERACTION OF ELECTROMAGNETIC FIELDS WITH BIOLOGIC MEMBRANES * , 1975, Annals of the New York Academy of Sciences.

[7]  W. R. Adey,et al.  203 - Possible Mechanisms of Weak Electromagnetic Field Coupling in Brain Tissue☆ , 1978 .

[8]  W. R. Adey,et al.  Models of long‐range order in cerebral macromolecules: Effects of sub‐ELF and of modulated VHF and UHF fields , 1979 .

[9]  J. Elder,et al.  Induction of calcium-ion efflux from brain tissue by radio-frequency radiation: Effects of modulation frequency and field strength , 1979 .

[10]  J. Elder,et al.  Induction of calcium-ion efflux from brain tissue by radiofrequency radiation: effect of sample number and modulation frequency on the power-density window. , 1980, Bioelectromagnetics.

[11]  D. House,et al.  Calcium-ion efflux from brain tissue: power-density versus internal field-intensity dependencies at 50-MHz RF radiation. , 1980, Bioelectromagnetics.

[12]  M. F. Sultan,et al.  Hyperthermic enhancement of antibody-complement cytotoxicity against normal mouse B lymphocytes and its relation to capping. , 1983, Radiation research.

[13]  M. F. Sultan,et al.  Effects of microwaves and hyperthermia on capping of antigen-antibody complexes on the surface of normal mouse B lymphocytes. , 1983, Bioelectromagnetics.