Objectively measured sperm motility and sperm head morphometry in boars (Sus scrofa): relation to fertility and seminal plasma growth factors.

This study was conducted to investigate the relationships between results of computer-assisted semen analysis (spermatozoal motility and sperm head morphometry) and fertility of boars. In addition, concentrations of insulin-like growth factor (IGF)-I and IGF-II in seminal plasma were determined. The nonreturn rate (NRR) and the number of live-born piglets were compatible with the requirements of artificial insemination for all boars included in this study. Semen samples of 12 boars (Pietrain; 3 ejaculates each) were evaluated for spermatozoal motility and sperm head dimensions using computer-assisted methods. Native semen samples were centrifuged, and seminal plasma was frozen at -20 degrees C until assayed for IGF-I and IGF-II by specific radioimmunoassays. Spermatozoa of boars with a higher NRR (>86%) had a significantly slower average velocity of motile spermatozoa when compared with that of boars with an NRR below 86%. High-fertility boars (NRR > 86%) had significantly smaller sperm heads than did boars with an NRR below 86%, and their sperm heads were less elongated. Substantial concentrations of IGF-I (8.4-22.2 ng/mL) and IGF-II (12.1-19.8 ng/mL) could be measured in porcine seminal plasma; however, there was no correlation between IGF levels and semen parameters or individual fertility.

[1]  C. Gravance,et al.  Automated sperm morphometry analysis (ASMA) in the rabbit. , 1995, Journal of andrology.

[2]  W. Holt,et al.  Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. , 1997, Journal of andrology.

[3]  R. Stolla,et al.  Influence of Random Factors and Two Different Staining Procedures on Computer‐assisted Sperm Head Morphometry in Bulls , 1999 .

[4]  J. Dadoune,et al.  Correlation Between Defects in Chromatin Condensation of Human Spermatozoa Stained by Aniline Blue and Semen Characteristics , 2009, Andrologia.

[5]  M. Sirard,et al.  Premature capacitation of bovine spermatozoa is initiated by cryopreservation. , 1997, Journal of andrology.

[6]  S. L. Gray,et al.  Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. , 1998, Biology of reproduction.

[7]  C. Bondy,et al.  Anatomy of the insulin-like growth factor system in the human testis. , 1993, Fertility and sterility.

[8]  C. Gravance,et al.  Standardization of specimen preparation, staining, and sampling methods improves automated sperm-head morphometry analysis. , 1993, Fertility and sterility.

[9]  C H Li,et al.  Identification of insulin-like growth factor-II in human seminal and follicular fluids. , 1986, Biochemical and biophysical research communications.

[10]  P. Casey,et al.  Effects of cryopreservation on bull sperm head morphometry. , 1998, Journal of andrology.

[11]  R. Foote,et al.  Quantification of bull sperm characteristics measured by computer-assisted sperm analysis (CASA) and the relationship to fertility. , 1998, Theriogenology.

[12]  P. Casey,et al.  Computer automated morphometric analysis of bull sperm heads. , 1996, Theriogenology.

[13]  B. Breier,et al.  Radioimmunoassays for IGFs and IGFBPs. , 1994, Growth regulation.

[14]  H. Glander,et al.  Andrology: Insulin-like growth factor-I and α2-macroglobulin in seminal plasma correlate with semen quality , 1996 .

[15]  A. Hoeflich,et al.  Insulin-like growth factors and IGF-binding proteins in bovine seminal plasma. , 1999, Domestic animal endocrinology.

[16]  C. Lombard,et al.  Sperm morphology: assessing the agreement between the manual method (strict criteria) and the sperm morphology analyzer IVOS. , 1995, Fertility and sterility.

[17]  H. Orskov,et al.  Growth hormone treatment of subfertile males. , 1996, Fertility and sterility.

[18]  B. Ball,et al.  Morphometry of stallion spermatozoa by computer-assisted image analysis. , 1995, Theriogenology.

[19]  C. Lombard,et al.  A prospective study on the predictive value of normal sperm morphology as evaluated by computer (IVOS). , 1996, Fertility and sterility.

[20]  S. Blottner,et al.  Chromatin condensation in cat spermatozoa during epididymal transit as studied by aniline blue and acridine orange staining , 2009, Andrologia.

[21]  P. Casey,et al.  Computer-assisted sperm head morphometry analysis (ASMA) of cryopreserved ram spermatozoa. , 1998, Theriogenology.

[22]  P. Casey,et al.  Quantification of normal head morphometry of stallion spermatozoa. , 1996, Journal of reproduction and fertility.

[23]  P. Casey,et al.  Computer automated sperm head morphometry analysis (ASMA) of goat spermatozoa. , 1995, Theriogenology.

[24]  F A Lacquet,et al.  Slide preparation and staining procedures for reliable results using computerized morphology. , 1996, Archives of andrology.

[25]  W. Holt,et al.  Choice of operating conditions to minimize sperm subpopulation sampling bias in the assessment of boar semen by computer-assisted semen analysis. , 1996, Journal of andrology.

[26]  R. G. Saacke,et al.  Chromatin structural changes in sperm after scrotal insulation of Holstein bulls. , 1997, Journal of andrology.

[27]  C. Soler,et al.  Computer assisted morphometric analysis of ram sperm heads: evaluation of different fixative techniques. , 1998, Theriogenology.

[28]  D. Evenson,et al.  Bull sperm head morphometry related to abnormal chromatin structure and fertility. , 1996, Cytometry.

[29]  P. Casey,et al.  Morphometric differences in sperm head dimensions of fertile and subfertile stallions. , 1997, Theriogenology.

[30]  W Holt,et al.  Reproducibility of computer-aided semen analysis: comparison of five different systems used in a practical workshop. , 1994, Fertility and sterility.