Modeling the depth-sectioning effect in reflection-mode dynamic speckle-field interferometric microscopy.

Unlike most optical coherence microscopy (OCM) systems, dynamic speckle-field interferometric microscopy (DSIM) achieves depth sectioning through the spatial-coherence gating effect. Under high numerical aperture (NA) speckle-field illumination, our previous experiments have demonstrated less than 1 μm depth resolution in reflection-mode DSIM, while doubling the diffraction limited resolution as under structured illumination. However, there has not been a physical model to rigorously describe the speckle imaging process, in particular explaining the sectioning effect under high illumination and imaging NA settings in DSIM. In this paper, we develop such a model based on the diffraction tomography theory and the speckle statistics. Using this model, we calculate the system response function, which is used to further obtain the depth resolution limit in reflection-mode DSIM. Theoretically calculated depth resolution limit is in an excellent agreement with experiment results. We envision that our physical model will not only help in understanding the imaging process in DSIM, but also enable better designing such systems for depth-resolved measurements in biological cells and tissues.

[1]  Mingguang Shan,et al.  Statistical dispersion relation for spatially broadband fields. , 2016, Optics letters.

[2]  Alberto Diaspro,et al.  Pushing phase and amplitude sensitivity limits in interferometric microscopy. , 2016, Optics letters.

[3]  Gabriel Popescu,et al.  Solving inverse scattering problems in biological samples by quantitative phase imaging , 2016 .

[4]  Nathan D. Shemonski,et al.  Computational high-resolution optical imaging of the living human retina , 2015, Nature Photonics.

[5]  Zahid Yaqoob,et al.  Dynamic speckle illumination wide-field reflection phase microscopy. , 2014, Optics letters.

[6]  G. Popescu,et al.  Inverse scattering solutions using low-coherence light. , 2014, Optics letters.

[7]  Brandon Redding,et al.  Full-field interferometric confocal microscopy using a VCSEL array. , 2014, Optics letters.

[8]  Tan H. Nguyen,et al.  Diffraction phase microscopy: principles and applications in materials and life sciences , 2014 .

[9]  S. D. Babacan,et al.  White-light diffraction tomography of unlabelled live cells , 2014, Nature Photonics.

[10]  Yongjin Sung,et al.  Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy , 2012, PloS one.

[11]  Wonshik Choi,et al.  Full-field and single-shot quantitative phase microscopy using dynamic speckle illumination. , 2011, Optics letters.

[12]  S. Kou,et al.  Image formation in holographic tomography: high-aperture imaging conditions. , 2009, Applied optics.

[13]  M. Gustafsson,et al.  Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. , 2008, Biophysical journal.

[14]  Daniel L Marks,et al.  Interferometric Synthetic Aperture Microscopy , 2007, OFC/NFOEC 2008 - 2008 Conference on Optical Fiber Communication/National Fiber Optic Engineers Conference.

[15]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[16]  A. Schierloh,et al.  Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain , 2007, Nature Methods.

[17]  Francesco Pampaloni,et al.  Life Sciences Require the Third Dimension This Review Comes from a Themed Issue on Cell Structure and Dynamics Edited Modern Three-dimensional Microscopy Spim Technology for Three-dimensional Cell Culture , 2022 .

[18]  J. Lichtman,et al.  Optical sectioning microscopy , 2005, Nature Methods.

[19]  F. Del Bene,et al.  Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy , 2004, Science.

[20]  A. Fercher,et al.  Optical coherence tomography - principles and applications , 2003 .

[21]  Michael G. Somekh,et al.  Wide field amplitude and phase confocal microscope with speckle illumination , 2000 .

[22]  B R Masters,et al.  Two-photon excitation fluorescence microscopy. , 2000, Annual review of biomedical engineering.

[23]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[24]  G. Ripandelli,et al.  Optical coherence tomography. , 1998, Seminars in ophthalmology.

[25]  T. Wilson,et al.  Method of obtaining optical sectioning by using structured light in a conventional microscope. , 1997, Optics letters.

[26]  Colin J. R. Sheppard,et al.  Three-dimensional coherent transfer function in a reflection-mode confocal scanning microscope , 1991 .

[27]  W. Denk,et al.  Two-photon laser scanning fluorescence microscopy. , 1990, Science.

[28]  T. Wilson,et al.  Optical sectioning in confocal fluorescent microscopes , 1989 .

[29]  D. Agard,et al.  Fluorescence microscopy in three dimensions. , 1989, Methods in cell biology.

[30]  M. Minsky.  Memoir on inventing the confocal scanning microscope , 1988 .

[31]  D. Agard.  Optical sectioning microscopy: cellular architecture in three dimensions. , 1984, Annual review of biophysics and bioengineering.

[32]  G E Sommargren,et al.  Optical heterodyne profilometry. , 1981, Applied optics.

[33]  E. Wolf.  Three-dimensional structure determination of semi-transparent objects from holographic data , 1969 .