Molecular analysis of the duck plague virus US10 gene and its encoding protein

In the past years, a number of conventional and advanced bioinformatics methods have been employed for predicting gene structure and function. Here, we used many bioinformatics softwares to analyse the duck plague virus US10 gene (GenBank accession number: EU195084). The results indicated that this gene encoded an estimated 322 putative protein, contained the conserved domain of the Gene66 (IR5) protein. The protein found many functional sites, but without any transmembrane region and signal peptide. Secondary structure prediction showed that random coil (52.48%) and alpha helix (44.10%) had a high proportion. Analysis of the antigenicity indicated that in the position of 28-44, 100-120 and 302-322 amino acids distributed the most likely epitopes. Phylogenetic tree of the amino acids sequences showed this gene had a closer evolutionary relationship with Mardivirus. These results provided rational data to elucidate biological function and physiological features of the gene and its encoding protein.

[1]  B. Kingham,et al.  The genome of herpesvirus of turkeys: comparative analysis with Marek's disease viruses. , 2001, The Journal of general virology.

[2]  R. Jia,et al.  Complete Genomic Sequence of Chinese Virulent Duck Enteritis Virus , 2012, Journal of Virology.

[3]  R. Diefenbach,et al.  Determination of Interactions between Tegument Proteins of Herpes Simplex Virus Type 1 , 2005, Journal of Virology.

[4]  B. Panigrahy,et al.  An outbreak of duck viral enteritis (duck plague) in domestic Muscovy ducks (Cairina moschata domesticus) in Illinois. , 2001, Avian diseases.

[5]  E. Emini,et al.  Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide , 1985, Journal of virology.

[6]  B. A. Jameson,et al.  The antigenic index: a novel algorithm for predicting antigenic determinants , 1988, Comput. Appl. Biosci..

[7]  T. Mettenleiter.  Herpesvirus Assembly and Egress , 2002, Journal of Virology.

[8]  T. Compton,et al.  Human Cytomegalovirus Glycoprotein B Is Required for Virus Entry and Cell-to-Cell Spread but Not for Virion Attachment, Assembly, or Egress , 2009, Journal of Virology.

[9]  A. Baiker,et al.  Mutational Analysis of the Repeated Open Reading Frames, ORFs 63 and 70 and ORFs 64 and 69, of Varicella-Zoster Virus , 2001, Journal of Virology.

[10]  Bo Ma,et al.  Molecular analysis of US10, S3, and US2 in duck enteritis virus , 2009, Virus Genes.

[11]  Yulong Wang,et al.  Determinants of antigenicity and specificity in immune response for protein sequences , 2011, BMC Bioinformatics.

[12]  W. Newcomb,et al.  Herpesvirus capsid assembly: insights from structural analysis. , 2011, Current opinion in virology.

[13]  Jing Wu,et al.  Molecular characterization of the genome of duck enteritis virus. , 2009, Virology.

[14]  M. Huebner,et al.  Transcriptional profiling of Marek’s disease virus genes during cytolytic and latent infection , 2008, Virus Genes.

[15]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[16]  M. S. Hofstad,et al.  Diseases of poultry , 1969 .

[17]  F. Salguero,et al.  Histopathological and ultrastructural changes associated with herpesvirus infection in waterfowl , 2002, Avian pathology : journal of the W.V.P.A.

[18]  Shengwang Liu,et al.  Different linkages in the long and short regions of the genomes of duck enteritis virus Clone-03 and VAC Strains , 2011, Virology Journal.

[19]  L. Enquist,et al.  Proteomic Characterization of Pseudorabies Virus Extracellular Virions , 2011, Journal of Virology.

[20]  M. Sakaguchi,et al.  Construction of recombinant Marek's disease virus type 1 (MDV1) expressing the Escherichia coli lacZ gene as a possible live vaccine vector: the US10 gene of MDV1 as a stable insertion site. , 1994, Vaccine.

[21]  H. Friedman,et al.  Herpes Simplex Virus Type 1 Glycoprotein E Mediates Retrograde Spread from Epithelial Cells to Neurites , 2009, Journal of Virology.

[22]  T. Daikoku,et al.  The product of the US10 gene of herpes simplex virus type 1 is a capsid/tegument-associated phosphoprotein which copurifies with the nuclear matrix. , 1997, The Journal of general virology.

[23]  R. E. Gough,et al.  Duck virus enteritis in Great Britain, 1980 to 1989 , 1990, Veterinary Record.

[24]  J. Maniloff,et al.  Virus taxonomy : eighth report of the International Committee on Taxonomy of Viruses , 2005 .

[25]  Barbara J. Kelly,et al.  Functional roles of the tegument proteins of herpes simplex virus type 1. , 2009, Virus research.

[26]  Huanchun Chen,et al.  Characterization of the genes encoding complete US10, SORF3, and US2 proteins from duck enteritis virus , 2009, Virus Genes.

[27]  P. Karplus,et al.  Prediction of chain flexibility in proteins , 1985, Naturwissenschaften.

[28]  R. N. Harty,et al.  Identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger. , 1992, Virology.

[29]  Nimrod D. Rubinstein,et al.  Computational characterization of B-cell epitopes. , 2008, Molecular immunology.

[30]  D. Shukla,et al.  Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry , 2009, The FEBS journal.

[31]  K. Bieńkowska-Szewczyk,et al.  Human antibodies to herpes simplex virus type 1 glycoprotein C are neutralizing and target the heparan sulfate-binding domain. , 2010, Virology.

[32]  B. Klupp,et al.  Herpesvirus assembly: a tale of two membranes. , 2006, Current opinion in microbiology.

[33]  M. Beer,et al.  Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains. , 2011, Virus research.

[34]  H. Ploegh,et al.  The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation , 2010, The Journal of experimental medicine.

[35]  K. Schat,et al.  Latency Sites and Reactivation of Duck Enteritis Virus , 2002, Avian diseases.