Ellagic acid modulates sodium valproate induced reproductive toxicity in male Wistar rats.

This study evaluated the protective effect of ellagic acid on sodium valproate-induced sperm abnormalities in male Wistar rats. A total of 30 rats were grouped into five groups, each having 6 animals. Vehicle, sodium valproate (400 mg/kg) and ellagic acid (10, 25, 50 mg/kg) were given orally from day 1 to day 7, and ellagic acid was continued for 3 more days. On day fourteen, animals were sacrificed and the different parameters were recorded. There was a significant decrease in the sperm count and sperm motility after the exposure to sodium valproate. The percentage of abnormal sperms increased in a dose-dependent manner. The histopathological examination revealed that sodium valproate had caused degeneration and desquamation of germinal cells in the epithelium and also showed a decrease in the Johnsen's scoring. Ellagic acid provided partial protection at the doses of 10 and 25 mg/kg and complete protection at 50 mg/kg, against sodium valproate induced testicular and spermatozoal damage.

[1]  J. Mountz,et al.  Advances in the diagnosis and treatment of epilepsy. , 2012, Seminars in nuclear medicine.

[2]  D. Erdogan,et al.  Effects of chronic treatment with valproate and oxcarbazepine on testicular development in rats , 2011, Seizure.

[3]  M. Sönmez,et al.  Toxic effect of cyclophosphamide on sperm morphology, testicular histology and blood oxidant-antioxidant balance, and protective roles of lycopene and ellagic acid. , 2010, Basic & clinical pharmacology & toxicology.

[4]  V. Paul,et al.  Reproductive toxicity of sodium valproate in male rats , 2010, Indian journal of pharmacology.

[5]  A. Cansu.  Antiepileptic drugs and hormones in children , 2010, Epilepsy Research.

[6]  M. Sönmez,et al.  Attenuation of cyclosporine A-induced testicular and spermatozoal damages associated with oxidative stress by ellagic acid. , 2010, International immunopharmacology.

[7]  C. Girish,et al.  Hepatoprotective activity of picroliv, curcumin and ellagic acid compared to silymarin on paracetamol induced liver toxicity in mice , 2009, Fundamental & clinical pharmacology.

[8]  C. Girish,et al.  Drug development for liver diseases: focus on picroliv, ellagic acid and curcumin , 2008, Fundamental & clinical pharmacology.

[9]  M. Sönmez,et al.  Improvement of cisplatin-induced injuries to sperm quality, the oxidant-antioxidant system, and the histologic structure of the rat testis by ellagic acid. , 2008, Fertility and sterility.

[10]  K. Narayana.  An aminoglycoside antibiotic gentamycin induces oxidative stress, reduces antioxidant reserve and impairs spermatogenesis in rats. , 2008, The Journal of toxicological sciences.

[11]  K. Narayana,et al.  An organophosphate insecticide methyl parathion (o- o- dimethyl o-4-nitrophenyl phosphorothioate) induces cytotoxic damage and tubular atrophy in the testis despite elevated testosterone level in the rat. , 2006, The Journal of toxicological sciences.

[12]  M. Päivänsalo,et al.  Effect of epilepsy and antiepileptic drugs on male reproductive health , 2004, Neurology.

[13]  G. A. Soliman,et al.  Effects of antiepileptic drugs carbamazepine and sodium valproate on fertility of male rats. , 1999, DTW. Deutsche tierarztliche Wochenschrift.

[14]  K. Veitch,et al.  Valproate and mitochondria. , 1993, Biochemical pharmacology.

[15]  E. J. Read,et al.  Correlation of sperm motion parameters with fertility in rats treated subchronically with epichlorohydrin. , 1991, Journal of andrology.

[16]  F. M. Johnson,et al.  Translocation and sperm abnormality assays in mouse spermatogonia treated with procarbazine. , 1979, Mutation research.

[17]  H. Broeker.  [Pharmacotherapy of epilepsies]. , 1988, Zeitschrift fur arztliche Fortbildung.

[18]  M. Meistrich,et al.  Damaging effects of fourteen chemotherapeutic drugs on mouse testis cells. , 1982, Cancer research.

[19]  S. Johnsen.  Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. , 1970, Hormones.