Circadian rhythms in the release of vasoactive intestinal polypeptide and arginine-vasopressin in organotypic slice culture of rat suprachiasmatic nucleus

Temporal profiles of the amount of vasoactive intestinal polypeptide (VIP) were examined in the medium of organotypic suprachiasmatic nucleus (SCN) slice cultures over a 2-day period. Arginine-vasopressin (AVP) level was also measured in the same medium. The slices of the SCN were obtained from 7-8-day-old rats and cultured individually in tubes on a roller drum for 14 days. The VIP amount in the medium of SCN culture showed a circadian rhythm with a approximately 22-h period. Circadian rhythms with identical periods were also observed in AVP amount of the same culture. However, the peak time of the VIP rhythm was slightly ahead of that of the AVP rhythm. Furthermore, the total VIP amount in the medium over a 24-h period was six times as large as that of AVP. These results suggest that there is a circadian rhythm of VIP which is released from the ventrolateral SCN.

[1]  R. Buijs,et al.  A daily rhythm in behavioral vasopressin sensitivity and brain vasopressin concentrations , 1985, Neuroscience Letters.

[2]  M. Jouvet,et al.  Daily variations in concentration of vasoactive intestinal polypeptide immunoreactivity in discrete brain areas of the rat , 1991, Brain Research.

[3]  D. Earnest,et al.  Circadian vasopressin release from perifused rat suprachiasmatic explants in vitro: effects of acute stimulation , 1987, Brain Research.

[4]  A. Pol The hypothalamic suprachiasmatic nucleus of rat: Intrinsic anatomy , 1980 .

[5]  S. T. Inouye,et al.  Photic regulation of peptides located in the ventrolateral subdivision of the suprachiasmatic nucleus of the rat: daily variations of vasoactive intestinal polypeptide, gastrin-releasing peptide, and neuropeptide Y , 1993, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[6]  E. Stopa,et al.  Localization of vasoactive intestinal peptide and peptide histidine isoleucine immunoreactivity and mRNA within the rat suprachiasmatic nucleus. , 1988, Brain research.

[7]  S. Puglisi‐Allegra,et al.  Circadian variations of noradrenaline, 5-hydroxytryptamine and dopamine in specific brain areas of C57B1/6 and BALB/c mice , 1982, Brain Research.

[8]  H. Heller,et al.  A serotonin agonist phase-shifts the circadian clock in the suprachiasmatic nuclei in vitro , 1990, Brain Research.

[9]  H. Okamura,et al.  Vasoactive intestinal peptide (VIP)-like immunoreactive neurons located in the rat suprachiasmatic nucleus receive a direct retinal projection , 1989, Neuroscience Letters.

[10]  S. Shibata,et al.  Influence of environmental light-dark cycle and enucleation on activity of suprachiasmatic neurons in slice preparations , 1984, Brain Research.

[11]  H. Nagasaki,et al.  A circadian rhythm of somatostatin messenger RNA levels, but not of vasoactive intestinal polypeptide/peptide histidine isoleucine messenger RNA levels in rat suprachiasmatic nucleus , 1992, Molecular and Cellular Neuroscience.

[12]  Sumio Takahashi,et al.  Circadian changes in arginine vasopressin level in the suprachiasmatic nuclei in the rat , 1991, Neuroscience Letters.

[13]  Steven M. Reppert,et al.  The hypothalamic suprachiasmatic nuclei: Circadian patterns of vasopressin secretion and neuronal activity in vitro , 1987, Brain Research Bulletin.

[14]  S. T. Inouye,et al.  Circadian rhythms of vasopressin content in the suprachiasmatic nucleus of the rat. , 1992, Neuroreport.

[15]  M. Mirmiran,et al.  Circadian rhythms in spontaneous neuronal discharges of the cultured suprachiasmatic nucleus , 1990, Brain Research.

[16]  W. Young,et al.  The diurnal rhythm in vasopressin V1a receptor expression in the suprachiasmatic nucleus is not dependent on vasopressin. , 1993, Endocrinology.

[17]  K. Shinohara,et al.  Circadian rhythms of somatostatin-immunoreactivity in the suprachiasmatic nucleus of the rat , 1991, Neuroscience Letters.

[18]  N. Koibuchi,et al.  Circadian rhythms of vasopressin release in primary cultures of rat suprachiasmatic nucleus , 1993, Brain Research.

[19]  N. Murakami,et al.  Long-term cultured neurons from rat suprachiasmatic nucleus retain the capacity for circadian oscillation of vasopressin release , 1991, Brain Research.

[20]  Shigenori Watanabe,et al.  Phase-resetting efect of 8-OH-DPAT, a serotonin1A receptor agonist, on the circadian rhythm of firing rate in the rat suprachiasmatic nuclei in vitro , 1992, Brain Research.

[21]  J. Meijer,et al.  Neurophysiology of the suprachiasmatic circadian pacemaker in rodents. , 1989, Physiological reviews.

[22]  H. Okamura,et al.  Vasoactive intestinal peptide immunoreactive neurons in the rat suprachiasmatic nucleus demonstrate diurnal variation , 1989, Brain Research.

[23]  S. Hisano,et al.  Differential immunolabeling for electron microscopy of diverse peptidergic neurons. , 1987, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  K. Chihara,et al.  Suprachiasmatic nucleus neurons immunoreactive for vasoactive intestinal polypeptide have synaptic contacts with axons immunoreactive for neuropeptide Y: An immunoelectron microscopic study in the rat , 1988, Neuroscience Letters.

[25]  J. Jacklet,et al.  Circadian Rhythm: Population of Interacting Neurons , 1971, Science.

[26]  T. Deguchi,et al.  Entrainment of the circadian rhythms of blinded infant rats by nursing mothers , 1983, Physiology & Behavior.

[27]  S. Reppert,et al.  Neural regulation of the circadian vasopressin rhythm in cerebrospinal fluid: a pre-eminent role for the suprachiasmatic nuclei , 1985, The Journal of neuroscience : the official journal of the Society for Neuroscience.