Functional Microbial Communities in Hybrid Linear Flow Channel Reactors for Desulfurization of Tannery Effluent

Recent research has demonstrated that hybrid linear flow channel reactors (HLFCRs) can desulfurize tannery effluent via sulfate reduction and concurrent oxidation of sulfide to elemental sulfur. The reactors can be used to pre-treat tannery effluent to improve the efficiency of downstream anaerobic digestion and recover sulfur. This study was conducted to gain insight into the bacterial communities in HLFCRs operated in series and identify structure-function relationships. This was accomplished by interpreting the results obtained from amplicon sequencing of the 16S rRNA gene and quantification of the dissimilatory sulfite reducing (dsrB) gene. In an effort to provide a suitable inoculum, microbial consortia were harvested from saline estuaries and enriched. However, it was found that bioaugmentation was not necessary because native communities from tannery wastewater were selected over exogenous communities from the enriched consortia. Overall, Dethiosulfovibrio sp. and Petrimonas sp. were strongly selected (maximum relative abundances of 29% and 26%, respectively), while Desulfobacterium autotrophicum (57%), and Desulfobacter halotolerans (27%) dominated the sulfate reducing bacteria. The presence of elemental sulfur reducing genera such as Dethiosulfovibrio and Petrimonas is not desirable in HLFCRs, and strategies to counter their selection need to be considered to ensure efficiency of these systems for pre-treatment of tannery effluent.

[1]  Rinku Pramanick,et al.  Vaginal Dysbiotic Microbiome in Women With No Symptoms of Genital Infections , 2022, Frontiers in Cellular and Infection Microbiology.

[2]  O. Oyekola,et al.  Biological Desulfurization of Tannery Effluent Using Hybrid Linear Flow Channel Reactors , 2021, Water.

[3]  P. Welz,et al.  Anaerobic co-digestion of tannery effluents: Process optimisation for resource recovery, recycling and reuse in a circular bioeconomy , 2021, Process Safety and Environmental Protection.

[4]  O. Oyekola,et al.  Sulfate-reducing and methanogenic microbial community responses during anaerobic digestion of tannery effluent. , 2021, Bioresource technology.

[5]  Moonhyun Hwang,et al.  Bioaugmentation treatment of a novel microbial consortium for degradation of organic pollutants in tannery wastewater under a full-scale oxic process , 2021 .

[6]  C. Saiz-Jimenez,et al.  Dominance of Arcobacter in the white filaments from the thermal sulfidic spring of Fetida Cave (Apulia, southern Italy). , 2021, The Science of the total environment.

[7]  L. T. Fuess,et al.  Increasing salinity concentrations determine the long-term participation of methanogenesis and sulfidogenesis in the biodigestion of sulfate-rich wastewater. , 2021, Journal of environmental management.

[8]  O. Oyekola,et al.  Anaerobic Co-Digestion of Tannery and Slaughterhouse Wastewater for Solids Reduction and Resource Recovery: Effect of Sulfate Concentration and Inoculum to Substrate Ratio , 2021, Energies.

[9]  Jae-Hwan Yang.  Hydrogen sulfide removal technology: A focused review on adsorption and catalytic oxidation , 2021, Korean Journal of Chemical Engineering.

[10]  S. Thennadil,et al.  Sulphate-Reducing Bacteria’s Response to Extreme pH Environments and the Effect of Their Activities on Microbial Corrosion , 2021, Applied Sciences.

[11]  O. Oyekola,et al.  Anaerobic treatment of tannery wastewater in the context of a circular bioeconomy for developing countries , 2021 .

[12]  P. Buttigieg,et al.  Comparison of Two 16S rRNA Primers (V3–V4 and V4–V5) for Studies of Arctic Microbial Communities , 2021, Frontiers in Microbiology.

[13]  R. Heyer,et al.  The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses , 2020, Microorganisms.

[14]  Zhili He,et al.  Synergistic interactions of Desulfovibrio and Petrimonas for sulfate-reduction coupling polycyclic aromatic hydrocarbon degradation. , 2020, Journal of hazardous materials.

[15]  S. Harrison,et al.  Effect of hydraulic residence time on biological sulphate reduction and elemental sulphur recovery in a single-stage hybrid linear flow channel reactor , 2020 .

[16]  C. Apperson,et al.  Diversity and structure of the bacterial microbiome of the American dog tick, Dermacentor variabilis, is dominated by the endosymbiont Francisella , 2019, Symbiosis.

[17]  B. Jørgensen,et al.  The Biogeochemical Sulfur Cycle of Marine Sediments , 2019, Front. Microbiol..

[18]  O. Tuovinen,et al.  Isolation, characterization, and genome insights into an anaerobic sulfidogenic Tissierella bacterium from Cu-bearing coins. , 2019, Anaerobe.

[19]  T. Marais,et al.  Demonstration of simultaneous biological sulphate reduction and partial sulphide oxidation in a hybrid linear flow channel reactor , 2019, bioRxiv.

[20]  P. Welz,et al.  The effect of biogenic and chemically manufactured silver nanoparticles on the benthic bacterial communities in river sediments. , 2018, The Science of the total environment.

[21]  A. Willis.  Rarefaction, Alpha Diversity, and Statistics , 2017, bioRxiv.

[22]  M. Schmidt-Heydt,et al.  Physicochemical and microbial soil quality indicators as affected by the agricultural management system in strawberry cultivation using straw or black polyethylene mulching , 2017 .

[23]  B. Jørgensen,et al.  The marine sulfate reducer Desulfobacterium autotrophicum HRM2 can switch between low and high apparent half‐saturation constants for dissimilatory sulfate reduction , 2017, FEMS microbiology ecology.

[24]  J. Fuhrman,et al.  Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. , 2016, Environmental microbiology.

[25]  T. Langer,et al.  Description of Proteiniphilum saccharofermentans sp. nov., Petrimonas mucosa sp. nov. and Fermentimonas caenicola gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Proteiniphilum. , 2016, International journal of systematic and evolutionary microbiology.

[26]  Duu-Jong Lee,et al.  Characterization of a newly isolated strain Pseudomonas sp. C27 for sulfide oxidation: Reaction kinetics and stoichiometry , 2016, Scientific Reports.

[27]  R. Parsons,et al.  Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton , 2015 .

[28]  Mark C M van Loosdrecht,et al.  A review of biological sulfate conversions in wastewater treatment. , 2014, Water research.

[29]  S. Burton,et al.  Selection of Clostridium spp. in biological sand filters neutralizing synthetic acid mine drainage. , 2014, FEMS microbiology ecology.

[30]  Susan P. Holmes,et al.  Waste Not , Want Not : Why Rarefying Microbiome Data is Inadmissible . October 1 , 2013 , 2013 .

[31]  J. Kuever,et al.  Sulphur isotope fractionation during the reduction of elemental sulphur and thiosulphate by Dethiosulfovibrio spp. , 2012, Isotopes in environmental and health studies.

[32]  Changqing Zhao,et al.  Isolation of a sulfate reducing bacterium and its application in sulfate removal from tannery wastewater , 2011 .

[33]  B. Patel,et al.  Dethiosulfovibrio salsuginis sp. nov., an anaerobic, slightly halophilic bacterium isolated from a saline spring. , 2010, International journal of systematic and evolutionary microbiology.

[34]  R. Rabus,et al.  Substrate-Dependent Regulation of Carbon Catabolism in Marine Sulfate-Reducing Desulfobacterium autotrophicum HRM2 , 2010, Journal of Molecular Microbiology and Biotechnology.

[35]  R. Amann,et al.  Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide , 2009, Environmental microbiology.

[36]  A. Dey,et al.  BIOLOGICAL TREATMENT OF TANNERY WASTEWAT ER FOR SULFIDE REMOVAL , 2008 .

[37]  W. Whitman,et al.  Thermoanaerobacter sulfurigignens sp. nov., an anaerobic thermophilic bacterium that reduces 1 M thiosulfate to elemental sulfur and tolerates 90 mM sulfite. , 2007, International journal of systematic and evolutionary microbiology.

[38]  B. Borremans,et al.  DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria. , 2006, Journal of microbiological methods.

[39]  C. Emmerling,et al.  Methods for evaluating human impact on soil microorganisms based on their activity, biomass, and diversity in agricultural soils , 2006 .

[40]  B. Tindall,et al.  Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir. , 2005, International journal of systematic and evolutionary microbiology.

[41]  P. Rose,et al.  Tannery effluent as a carbon source for biological sulphate reduction. , 2004, Water research.

[42]  T. Anderson,et al.  Quantitative Isolation of Microbial DNA from Different Types of Soils of Natural and Agricultural Ecosystems , 2003, Microbiology.

[43]  J. S. van der Hoeven,et al.  Isolation of Desulfomicrobium orale sp. nov. and Desulfovibrio strain NY682, oral sulfate-reducing bacteria involved in human periodontal disease. , 2001, International journal of systematic and evolutionary microbiology.

[44]  F. Glöckner,et al.  Dethiosulfovibrio russensis sp. nov., Dethosulfovibrio marinus sp. nov. and Dethosulfovibrio acidaminovorans sp. nov., novel anaerobic, thiosulfate- and sulfur-reducing bacteria isolated from 'Thiodendron' sulfur mats in different saline environments. , 2001, International journal of systematic and evolutionary microbiology.

[45]  J. Kuever,et al.  Stable sulfur isotope fractionation during the reduction of thiosulfate by Dethiosulfovibrio russensis , 2000, Archives of Microbiology.

[46]  X. Guan,et al.  Relationship between dsDNA, chloroform labile C and ergosterol in soils of different organic matter contents and pH. , 2000 .

[47]  Michael Wagner,et al.  Phylogeny of Dissimilatory Sulfite Reductases Supports an Early Origin of Sulfate Respiration , 1998, Journal of bacteriology.

[48]  K. Brandt,et al.  Desulfobacter halotolerans sp. nov., a Halotolerant Acetate-Oxidizing Sulfate-Reducing Bacterium Isolated from Sediments of Great Salt Lake, Utah , 1997 .

[49]  J. Crolet,et al.  Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells. , 1997, International journal of systematic bacteriology.