Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars

Abstract Nine microsatellite markers (VVMD5, VVMD7, VVS2, ssrVrZAG21, ssrVrZAG47, ssrVrZAG62, ssrVrZAG64, ssrVrZAG79 and ssrVrZAG83) were chosen for the analysis of marker information content, the genetic structure of grapevine cultivar gene pools, and differentiation among grapevines sampled from seven European vine-growing regions (Greece, Croatia, North Italy, Austria and Germany, France, Spain and Portugal). The markers were found to be highly informative in all cultivar groups and therefore constitute a useful set for the genetic characterization of European grapevines. Similar and high levels of genetic variability were detected in all investigated grapevine gene pools. Genetic differentiation among cultivars from different regions was significant, even in the case of adjacent groups such as the Spanish and Portuguese cultivars. No genetic differentiation could be detected between vines with blue and white grapes, indicating that they have undergone the processes of cultivar development jointly. The observed genetic differentiation among vine-growing regions suggested that cultivars could possibly be assigned to their regions of origin according to their genotypes. This might allow one to determine the geographical origin of cultivars with an unknown background. The assignment procedure proved to work for cultivars from the higher differentiated regions, as for example from Austria and Portugal.

[1]  J. Bowers,et al.  Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). , 1996, Genome.

[2]  M. Morgante,et al.  PCR-amplified microsatellites as markers in plant genetics. , 1993, The Plant journal : for cell and molecular biology.

[3]  Ö. Wien.  Genotyping of grapevine and rootstock cultivars using microsatellite markers , 1998 .

[4]  François Rousset,et al.  GENEPOP (version 1.2): population genetic software for exact tests and ecumenicism , 1995 .

[5]  B. Weir,et al.  ESTIMATING F‐STATISTICS FOR THE ANALYSIS OF POPULATION STRUCTURE , 1984, Evolution; international journal of organic evolution.

[6]  F. Lefort,et al.  An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus , 1999 .

[7]  H. Steinkellner,et al.  The use of microsatellites for germplasm management in a Portuguese grapevine collection , 1999, Theoretical and Applied Genetics.

[8]  I. Stirling,et al.  Microsatellite analysis of population structure in Canadian polar bears , 1995, Molecular ecology.

[9]  A. Bowling,et al.  Validation of microsatellite markers for routine horse parentage testing. , 1997, Animal genetics.

[10]  A. von Haeseler,et al.  Paternity assessment and population subdivision in a natural population of the larger mouse‐eared bat Myotis myotis , 1997, Molecular Ecology.

[11]  F. Regner,et al.  Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. , 1999, Genome.

[12]  J. Goudet.  FSTAT (Version 1.2): A Computer Program to Calculate F-Statistics , 1995 .

[13]  P. Arctander,et al.  Population structure of African buffalo inferred from mtDNA sequences and microsatellite loci: high variation but low differentiation , 1998, Molecular ecology.

[14]  S. Wright,et al.  Genetical Structure of Populations , 1950, Nature.

[15]  M. Shriver,et al.  Microsatellite DNA variation and the evolution, domestication and phylogeography of taurine and zebu cattle (Bos taurus and Bos indicus). , 1997, Genetics.

[16]  F. Regner,et al.  Reconstruction of a grapevine pedigree by microsatellite analysis , 1998, Theoretical and Applied Genetics.

[17]  S. Hokanson,et al.  Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus×domestica borkh. core subset collection , 1998, Theoretical and Applied Genetics.

[18]  M. Nei.  Analysis of gene diversity in subdivided populations. , 1973, Proceedings of the National Academy of Sciences of the United States of America.