Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection

A simple and accurate ion chromatography (IC) method with pulsed amperometric detection (PAD) was proposed for the determination of cyanide ion in urine, sweat, and saliva samples. The sample pretreatment relies on alkaline digestion and application of Dionex OnGuard II H cartridge. Under the optimized conditions, the method showed good linearity in the range of 1–100 μg/L for urine, 5–100 μg/L for saliva, and 3–100 μg/L for sweat samples with determination coefficients (R) > 0.992. Low detection limits (LODs) in the range of 1.8 μg/L, 5.1 μg/L, and 5.8 μg/L for urine, saliva, and sweat samples, respectively, and good repeatability (CV < 3%, n = 3) were obtained. The proposed method has been successfully applied to the analysis of human biological samples.

[1]  E. Aljuhani,et al.  Headspace sorptive solid phase microextraction (HS-SPME) combined with a spectrophotometry system: A simple glass devise for extraction and simultaneous determination of cyanide and thiocyanate in environmental and biological samples. , 2016, Talanta.

[2]  N. Takayama,et al.  Simultaneous determination of cyanide and thiocyanate in blood by ion chromatography with fluorescence and ultraviolet detection. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[3]  B. Ballantyne.  The influence of exposure route and species on the acute lethal toxicity and tissue concentrations of cyanide. , 1983, Developments in toxicology and environmental science.

[4]  Fina Petrova,et al.  World Health Organization , 1946, International Encyclopedia of Public Health.

[5]  J. Namieśnik,et al.  Determination of formaldehyde and cyanide ion in human nasal discharge by using simple spectrophotometric methods , 2012 .

[6]  K. Kudo,et al.  Determination of cyanide and thiocyanate in blood by gas chromatography and gas chromatography-mass spectrometry. , 1996, Journal of chromatography. B, Biomedical applications.

[7]  David A. Dzombak,et al.  Cyanide in water and soil: chemistry, risk and management. , 2005 .

[8]  B. Mégarbane,et al.  An unusual cause of severe lactic acidosis: cyanide poisoning after bitter almond ingestion , 2010, Intensive Care Medicine.

[9]  P. Krajewski,et al.  LC-MS/MS method development and validation for quantitative analyses of 2-aminothiazoline-4-carboxylic acid--a new cyanide exposure marker in post mortem blood. , 2016, Talanta.

[10]  Qiyang Zhang,et al.  A direct and rapid method to determine cyanide in urine by capillary electrophoresis. , 2015, Journal of chromatography. A.

[11]  C. Skinner,et al.  Cyanide quantification in post-mortem biological matrices by headspace GC-MS. , 2012, Forensic science international.

[12]  A. Gustavo González,et al.  A practical guide to analytical method validation, including measurement uncertainty and accuracy profiles , 2007 .

[13]  J. Rohrer,et al.  Direct determination of free cyanide in drinking water by ion chromatography with pulsed amperometric detection. , 2007, Journal of chromatography. A.

[14]  J. Namieśnik,et al.  Cyanides in the environment—analysis—problems and challenges , 2017, Environmental Science and Pollution Research.

[15]  P. Dasgupta,et al.  Recent developments in cyanide detection: a review. , 2010, Analytica chimica acta.

[16]  J. Namieśnik,et al.  Solid phase microextraction–comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry: a new tool for determining PAHs in airport runoff water samples , 2016 .

[17]  K. Hara,et al.  Rapid determination of cyanide in human plasma and urine by gas chromatography-mass spectrometry with two-step derivatization. , 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[18]  Diane M. Hinkens,et al.  The Analysis of Cyanide and its Breakdown Products in Biological Samples , 2010 .

[19]  J. Namieśnik,et al.  Application of ion chromatography for the determination of inorganic ions, especially thiocyanates in human saliva samples as biomarkers of environmental tobacco smoke exposure. , 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[20]  M. Colina,et al.  Determination of anions in human and animal tear fluid and blood serum by ion chromatography. , 1995, Journal of chromatography. A.