Peritoneal cell response during adhesion formation.

BACKGROUND We estimated a number of cell populations of peritoneal fluid in rats with experimentally developed peritoneal adhesions. MATERIALS AND METHODS The fluid was taken in standarized conditions at the first operation and during reoperation. Animals were divided into four groups. The first group was the control group (n = 20) on which the abdomen was opened and closed without any manipulations (group 1a), and the reoperation was done after 72 hr (group 1b). The other groups (2, 3, 4; n = 20 for each group) were operated, and scarification of the parietal peritoneum and serosa of the bowel was performed. The rats were reoperated after 24, 72, and 168 hr, respectively, after the first surgery. The peritoneal cavity was washed with the 0.9% saline solution. The number of the peritoneal adhesions and populations of the cells were counted. RESULTS The highest number of peritoneal adhesions was observed in the group of animals reoperated after 72 hr. After 72 and 168 hr, the higher number of mast cells (MC) and neutrophils was observed. The difference was statistically significant. The percentage of mast cells (MC) increased during the experiment. It was different from other cell populations which decreased after 168 hr. The highest change in number was observed for MC. The highest number of MC was observed on the seventh day after the laparotomy. CONCLUSIONS MC influence peritoneal adhesion formation and probably take part in adhesion remodeling. MC and neutrophils changed significantly after manipulations in the peritoneal cavity. Probably, they play an important role in peritoneal adhesion formation.

[1]  J. van den Born,et al.  Novel role for mast cells in omental tissue remodeling and cell recruitment in experimental peritoneal dialysis. , 2006, Journal of the American Society of Nephrology : JASN.

[2]  H. Redmond,et al.  Mast cells facilitate local VEGF release as an early event in the pathogenesis of postoperative peritoneal adhesions. , 2006, Surgery.

[3]  F. Levi-Schaffer,et al.  The role of mast cell-derived histamine in the closure of an in vitro wound , 1996, Inflammation Research.

[4]  K. Norrby.  Evidence of mast-cell histamine being mitogenic in intact tissue , 1985, Agents and Actions.

[5]  F. Levi-Schaffer,et al.  Mast cell dynamics and involvement in the development of peritoneal adhesions in the rat. , 2002, Life sciences.

[6]  W L Ledger,et al.  Peritoneal healing and adhesion formation/reformation. , 2001, Human reproduction update.

[7]  J. Campeau,et al.  Peritoneal repair and post-surgical adhesion formation. , 2001, Human reproduction update.

[8]  J. Wedemeyer,et al.  Roles of mast cells and basophils in innate and acquired immunity. , 2000, Current opinion in immunology.

[9]  A. Haney.  Identification of macrophages at the site of peritoneal injury: evidence supporting a direct role for peritoneal macrophages in healing injured peritoneum. , 2000, Fertility and sterility.

[10]  B. Klosterhalfen,et al.  Modulation of Pro- and Antifibrinolytic Properties of Human Peritoneal Mesothelial Cells by Transforming Growth Factor β1 (TGF- β1), Tumor Necrosis Factor α (TNF-α) and Interleukin β1 (IL-1β) , 1998, Thrombosis and Haemostasis.

[11]  J. Thompson.  Pathogenesis and Prevention of Adhesion Formation , 1998, Digestive Surgery.

[12]  B. Risberg,et al.  Characterization and fibrinolytic properties of mesothelial cells isolated from peritoneal lavage. , 1998, Scandinavian journal of clinical and laboratory investigation.

[13]  J. Garlick,et al.  Human mast cells activate fibroblasts: tryptase is a fibrogenic factor stimulating collagen messenger ribonucleic acid synthesis and fibroblast chemotaxis. , 1997, Journal of immunology.

[14]  B. Hylander,et al.  Tumor necrosis factor-α, interleukin-1β, and interleukin-1 receptor antagonist in dialysate and serum from patients on continuous ambulatory peritoneal dialysis , 1996 .

[15]  J. Langer,et al.  Mast cell mediators and peritoneal adhesion formation in the rat. , 1995, The Journal of surgical research.

[16]  F. Levi-Schaffer.  Mast cell/fibroblast interactions in health and disease. , 1995, Chemical immunology.

[17]  R. F. Morgan,et al.  Analysis of the kinetics of peritoneal adhesion formation in the rat and evaluation of potential antiadhesive agents. , 1995, Surgery.

[18]  H. Sawada,et al.  Human mast cells produce type VIII collagen in vivo. , 1994, International journal of experimental pathology.

[19]  A. Haney,et al.  The formation of coalescing peritoneal adhesions requires injury to both contacting peritoneal surfaces. , 1994, Fertility and sterility.

[20]  S. Lowry,et al.  Cytokine mediators of immunity and inflammation. , 1993, Archives of surgery.

[21]  T. Tamaya,et al.  Peritoneal Fluid lnterleukin‐1β and Tumor Necrosis Factor in Patients With Benign Gynecologic Disease , 1991, American journal of reproductive immunology.

[22]  D. Metcalfe,et al.  Murine mast cells synthesize basement membrane components. A potential role in early fibrosis. , 1991, The Journal of clinical investigation.

[23]  D. Olive,et al.  The nature of the intraperitoneal exudate associated with infertility: peritoneal fluid and serum lysozyme activity. , 1987, Fertility and sterility.

[24]  J. Hulka,et al.  Classification of adnexal adhesions: a proposal and evaluation of its prognostic value. , 1978, Fertility and sterility.

[25]  D. Milligan,et al.  Observations on the pathogenesis of peritoneal adhesions: a light and electron microscopical study , 1974, The British journal of surgery.

[26]  Raftery At.  Regeneration of parietal and visceral peritoneum: an electron microscopical study. , 1973 .