Morphological characterization of yeast by image analysis

A semiautomatic image analysis method, with minimal operator intervention, has been developed to characterize the morphology of yeast cells under the assumption that they have an ellipsoidic shape. The cells are observed by optical microscopy and the surface and the minor and major half‐axes of the projection of the ellipsoid on the image plane are determined. Using this method, yeast size distributions and population kinetics (single and budding cells, cell clusters) are determined during alcoholic fermentations. Combination of image analysis with a methylene blue viability test is examined but the staining procedure induces a change in the size of the cells. © 1993 John Wiley & Sons, Inc.

[1]  M. Pons,et al.  Application of quantitative image analysis to a mammalian cell line grown on microcarriers. , 1992, Biotechnology and bioengineering.

[2]  J. A. Valkenburg,et al.  A computer‐aided measuring system for the characterization of yeast populations combining 2D‐image analysis, electronic particle counter, and flow cytometry , 1992, Biotechnology and bioengineering.

[3]  L. Alberghina,et al.  Cell size modulation by CDC25 and RAS2 genes in Saccharomyces cerevisiae , 1989, Molecular and cellular biology.

[4]  G C Johnston,et al.  Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. , 1977, Experimental cell research.

[5]  P. Lord,et al.  Variability in individual cell cycles of Saccharomyces cerevisiae. , 1981, Journal of cell science.

[6]  P J Costello,et al.  Image analysis method for the rapid counting of Saccharomyces cerevisiae cells , 1985, Applied and environmental microbiology.

[7]  K. Luyben,et al.  The use of a Coulter Counter to investigate a mixed culture of Pichia stipitis and Saccharomyces cerevisiae , 1990 .

[8]  P. Barré,et al.  The use of a coulter counter to study the alcoholic fermentation in enological conditions , 1992 .

[9]  M. Okazaki,et al.  Cell aggregation and elongated cell morphology caused by a mutation conferring increased sensitivity to indole-3-acetic acid in the yeast, Saccharomyces cerevisiae , 1991 .

[10]  L. Hartwell,et al.  Asymmetrical division of Saccharomyces cerevisiae , 1980, Journal of bacteriology.

[11]  P. Lord,et al.  Dependency of size of Saccharomyces cerevisiae cells on growth rate , 1979, Journal of bacteriology.