Improved reconstructions and generalized filtered back projection for optical projection tomography.

Optical projection tomography (OPT) is a noninvasive imaging technique that enables imaging of small specimens (<1 cm), such as organs or animals in early developmental stages. In this paper, we present a set of computational methods that can be applied to the acquired data sets in order to correct for (a) unknown background or illumination intensity distributions over the field of view, (b) intensity spikes in single CCD pixels (so-called "hot pixels"), and (c) refractive index mismatch between the media in which the specimens are embedded and the environment. We have tested these correction methods using a variety of samples and present results obtained from Parhyale hawaiensis embedded in glycerol and in sea water. Successful reconstructions of fluorescence and absorption OPT images have been obtained for weakly scattering specimens embedded in media with nonmatched refractive index, thus advancing OPT toward routine in vivo imaging.

[1]  James Sharpe,et al.  In vitro whole-organ imaging: 4D quantification of growing mouse limb buds , 2008, Nature Methods.

[2]  Vasilis Ntziachristos,et al.  Noncontact optical tomography of turbid media. , 2003, Optics letters.

[3]  J. Vane,et al.  Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies , 2002 .

[4]  Ralph Weissleder,et al.  High throughput transmission optical projection tomography using low cost graphics processing unit. , 2009, Optics express.

[5]  Jorge Ripoll,et al.  Source intensity profile in noncontact optical tomography. , 2010, Optics letters.

[6]  James Sharpe,et al.  Fluorescence lifetime optical projection tomography , 2008, Journal of biophotonics.

[7]  Vasilis Ntziachristos,et al.  In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography , 2007, Nature Methods.

[8]  Improved fluorescence optical projection tomography reconstruction , 2008 .

[9]  M. Averof,et al.  Establishing genetic transformation for comparative developmental studies in the crustacean Parhyale hawaiensis , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J. H. Seldin,et al.  Hubble Space Telescope characterized by using phase-retrieval algorithms. , 1993, Applied optics.

[11]  Jorge Ripoll,et al.  Correction for specimen movement and rotation errors for in-vivo Optical Projection Tomography , 2010, Biomedical optics express.

[12]  M. Brady,et al.  Weighted filtered backprojection for quantitative fluorescence optical projection tomography , 2008, Physics in medicine and biology.

[13]  Bozena Kaminska,et al.  Transmission and fluorescence angular domain optical projection tomography of turbid media. , 2009, Applied optics.

[14]  Paul C. Lauterbur,et al.  Magnetic resonance zeugmatography , 1974 .

[15]  J. Sharpe.  Optical projection tomography. , 2004, Annual review of biomedical engineering.

[16]  Vasilis Ntziachristos,et al.  Normalized Born ratio for fluorescence optical projection tomography. , 2009, Optics letters.

[17]  Johnathon R. Walls,et al.  Correction of artefacts in optical projection tomography , 2005, Physics in medicine and biology.