Lipid Content and In Vitro Incorporation of Free Fatty Acids Into Lipids of Human Platelets: The Effect of Storage at 4°C

Lipid content and capacity to incorporate in vitro palmitate-1-14C and linoleate-1-14C into lipids was investigated in fresh and stored (4°C) human platelets. Cholesterol and phospholipids decreased 30% during storage for 6 days. Molar ratio of cholesterol to phospholipids and percentage distribution of individual phospholipids were similar in fresh and stored platelets. Palmitate bound to albumin was rapidly transferred by an energy-independent mechanism into a free fatty acid fraction of platelets. From there it was incorporated into glycerides and phospholipids, a process requiring energy. More palmitate than linoleate was incorporated into fatty acids and glycerides of fresh and stored platelets but linoleate exceeded palmitate in its incorporation into phosphatidylethanolamine. Storage of platelets produced the following changes: (1) Incorporation of palmitate into total lipids was significantly reduced but not that of linoleate. (2) Both palmitate and linoleate showed increased incorporation into phosphatidylethanolamine. (3) Incorporation of linoleate into free fatty acids and triglycerides and of palmitate into phosphatidylcholine was reduced.

[1]  M. Steiner,et al.  Incorporation of Acetate and Fatty Acids into Lipids of Rat Platelets 1 , 1971, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[2]  J. Hoak,et al.  Utilization of long-chain free fatty acids by human platelets. , 1970, The Journal of clinical investigation.

[3]  J. Doery,et al.  Energy metabolism in human platelets: interrelationship between glycolysis and oxidative metabolism. , 1970, Blood.

[4]  M. Steiner,et al.  Studies on lipid peroxides in platelets. I. Method of assay and effect of storage. , 1970, The Journal of laboratory and clinical medicine.

[5]  P. Majerus,et al.  Lipid metabolism in human platelets. , 1969, Series haematologica.

[6]  J. H. Jandl,et al.  The selective and conjoint loss of red cell lipids. , 1969, The Journal of clinical investigation.

[7]  D. Deykin,et al.  The incorporation of acetate and palmitate into lipids by human platelets. , 1968, The Journal of clinical investigation.

[8]  M. Ganoza,et al.  Studies on the phospholipid requirement of glucose 6-phosphatase. , 1968, The Journal of biological chemistry.

[9]  C. Winterbourn,et al.  Incorporation of [I-14C]palmitate into the lipids of bovine blood cells. , 1968, Biochimica et biophysica acta.

[10]  D. E. Green,et al.  Studies on the electron transfer system. LXV. Formation of membranes by purified cytochrome oxidase. , 1966, The Journal of biological chemistry.

[11]  C. P. Freeman,et al.  Complete separation of lipid classes on a single thin-layer plate. , 1966, Journal of lipid research.

[12]  V. P. Skipski,et al.  Separation of lipid classes by thin-layer chromatography. , 1965, Biochimica et biophysica acta.

[13]  E E WOODSIDE,et al.  LIPIDS OF HUMAN PLATELETS AND THEIR ACTION ON THE BLOOD COAGULATION PROCESS. , 1964, Blood.

[14]  G. S. Eadie,et al.  The Relation between Storage Time of Erythrocytes and Their Percentage Viability after Transfusion , 1963, Transfusion.

[15]  S. Fleischer,et al.  Studies of the electron transfer system. 47. The role of phospholipids in electron transfer. , 1962, The Journal of biological chemistry.

[16]  W. Dameshek,et al.  The viability of stored human platelets. , 1960, Blood.

[17]  P. Marks,et al.  Lipid synthesis in human leukocytes, platelets, and erythrocytes. , 1960, The Journal of biological chemistry.

[18]  R. Searcy,et al.  A new color reaction for the quantitation of serum cholesterol. , 1960, Clinica chimica acta; international journal of clinical chemistry.

[19]  G. Born,et al.  Changes in the distribution of phosphorus in platelet-rich plasma during clotting. , 1958, The Biochemical journal.

[20]  E. Cronkite,et al.  Morphology and enumeration of human blood platelets. , 1950, Journal of applied physiology.

[21]  A. Kuramoto,et al.  Energy metabolism of aggregating platelets. , 1971, Series haematologica.

[22]  A. Derksen,et al.  Pathways of fatty acid metabolism in human platelets. , 1970, The Journal of clinical investigation.

[23]  A. Marcus,et al.  Lipid composition of subcellular particles of human blood platelets. , 1969, Journal of lipid research.

[24]  J. Dodge,et al.  Composition of Phospholipids and of Phospholipid Fatty Acids and Aldehydes in Human Red Cells , 1967 .

[25]  C. K. Grantham,et al.  Cholesterol Esters of the Developing Liver: An Evaluation of the Flame Ionization Detector in Analyzing Tissue Fatty Acids , 1964 .

[26]  R. Gross,et al.  Über den Energiestoffwechsel normaler menschlicher Thrombozyten , 1959, Thrombosis and Haemostasis.

[27]  B. A. Pethica,et al.  The Physical Chemistry of Haemolysis and Bacteriolysis by Surface Active Agents and Antibiotics , 1955 .

[28]  H. H. Williams,et al.  THE LIPID DISTRIBUTION OF HUMAN PLATELETS IN HEALTH AND DISEASE. , 1939, The Journal of clinical investigation.