Evaluation of three selective chromogenic media, CHROMagar ESBL, CHROMagar CTX-M and CHROMagar KPC, for the detection of Klebsiella pneumoniae producing OXA-48 carbapenemase

Three selective chromogenic culture media (CHROMagars ESBL, CTX-M and KPC) were evaluated for their ability to support the growth of nine Klebsiella pneumoniae isolates producing OXA-48 carbapenemase in combination with other β-lactamases. CHROMagar ESBL and CHROMagar KPC were the most sensitive media, supporting growth of all isolates with a detection limit as low as < 100 CFU/ml. Five isolates failed to grow on CHROMagar CTX-M, and five were recovered on CHROMagar KPC only at counts > 106 CFU/ml. Both CHROMagar ESBL and CHROMagar KPC may be useful for enhanced isolation of K pneumoniae producing OXA-48-like carbapenemases.

[1]  N. Woodford,et al.  Characterization of Enterobacteriaceae producing OXA-48-like carbapenemases in the UK. , 2012, The Journal of antimicrobial chemotherapy.

[2]  V. Miriagou,et al.  Identification and screening of carbapenemase-producing Enterobacteriaceae. , 2012, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[3]  P. Nordmann,et al.  European dissemination of a single OXA-48-producing Klebsiella pneumoniae clone. , 2011, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[4]  P. Nordmann,et al.  Emergence of OXA-48-Producing Escherichia coli Clone ST38 in Fowl , 2011 .

[5]  A. Andremont,et al.  Risk of highly resistant bacteria importation from repatriates and travelers hospitalized in foreign countries: about the French recommendations to limit their spread. , 2011, Journal of travel medicine.

[6]  Y. Carmeli,et al.  Laboratory evaluation of the CHROMagar KPC medium for identification of carbapenem-nonsusceptible Enterobacteriaceae. , 2011, Diagnostic microbiology and infectious disease.

[7]  P. Nordmann,et al.  Emergence of OXA-48-Producing Escherichia coli Clone ST38 in France , 2011, Antimicrobial Agents and Chemotherapy.

[8]  M. Marre,et al.  Development of a Phenotypic Method for Detection of Fecal Carriage of OXA-48-Producing Enterobacteriaceae after Incidental Detection from Clinical Specimen , 2011, Journal of Clinical Microbiology.

[9]  Y. Carmeli,et al.  Laboratory and Clinical Evaluation of Screening Agar Plates for Detection of Carbapenem-Resistant Enterobacteriaceae from Surveillance Rectal Swabs , 2011, Journal of Clinical Microbiology.

[10]  A. Antoniadou,et al.  Evaluation of CHROMagar™ KPC for the detection of carbapenemase-producing Enterobacteriaceae in rectal surveillance cultures. , 2011, International journal of antimicrobial agents.

[11]  T. Walsh.  Emerging carbapenemases: a global perspective. , 2010, International journal of antimicrobial agents.

[12]  Neil Woodford,et al.  Comparison of BD Phoenix, Vitek 2, and MicroScan Automated Systems for Detection and Inference of Mechanisms Responsible for Carbapenem Resistance in Enterobacteriaceae , 2010, Journal of Clinical Microbiology.

[13]  J. Turton,et al.  PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets. , 2010, Journal of medical microbiology.

[14]  P. Nordmann,et al.  Use of ChromID Extended-Spectrum β-Lactamase Medium for Detecting Carbapenemase-Producing Enterobacteriaceae , 2010, Journal of Clinical Microbiology.

[15]  G. Arlet,et al.  Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. , 2010, The Journal of antimicrobial chemotherapy.

[16]  E. Liébana,et al.  Evaluation of CHROMagar CTX, a novel medium for isolating CTX-M-ESBL-positive Enterobacteriaceae while inhibiting AmpC-producing strains. , 2008, The Journal of antimicrobial chemotherapy.

[17]  Z. Samra,et al.  Evaluation of CHROMagar KPC for Rapid Detection of Carbapenem-Resistant Enterobacteriaceae , 2008, Journal of Clinical Microbiology.

[18]  P. Nordmann,et al.  Emergence of Oxacillinase-Mediated Resistance to Imipenem in Klebsiella pneumoniae , 2004, Antimicrobial Agents and Chemotherapy.

[19]  G. Robertson,et al.  Evaluation of CHROMagar Orientation for differentiation and presumptive identification of gram-negative bacilli and Enterococcus species , 1996, Journal of clinical microbiology.