Effect of ball milling-assisted glycosylation modification on the structure and foaming property of egg white protein.

The effect of different glycosylation degrees on molecular structure and foaming property of egg white protein (EWP) was investigated using ball milling-assisted glycosylation. The results showed the foaming ability (FA) and foam stability (FS) of EWP improved when the degree of glycosylation was increased. In particular, FA of ball milling-assisted glycosylation of EWP enhanced by 39.9% and 28.8%, and the FS increased by 28.7% and 24.0% compared with EWP and ball milling egg white protein (BE) at 150 min of reaction. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis could reflect the grafting degree of EWP and glucose molecules from the side. When EWP was fully grafted with glucose, endogenous fluorescence and free sulfhydryl groups indicated that tertiary structure of EWP was depolymerized, and Fourier transform infrared spectroscopy showed the secondary structure tended to change from order to disorder. The results of this study indicated that ball milling-assisted glycosylation modification was a practical method to improve the foaming property of EWP. PRACTICAL APPLICATION: EWP has great FA and FS, making it indispensable in the baking industry. In this study, ball milling-assisted glycosylation was used to improve the foaming property of EWP, and the molecular structure of EWP with different degrees of glycosylation was fully resolved. The results demonstrated that ball milling, as a physical pretreatment, can fully unfold the structure of EWP. When sugar molecules were fully grafted, the particle size of EWP reduced, solubility increased, and the stability of system improved, thus enhancing the foaming property of EWP. The results can provide theoretical basis for improving the foaming property of EWP and provide a reference value for its industrial application.

[1]  Hongbo Song,et al.  Effect of nano eggshell calcium on the structure, physicochemical, and gel properties of threadfin bream (Nemipterus virgatus) actomyosin , 2021 .

[2]  Hongbo Song,et al.  Mechanism of differences in characteristics of thick/thin egg whites during storage: Physicochemical, functional and molecular structure characteristics analysis. , 2021, Food chemistry.

[3]  F. Geng,et al.  Ovomucin may be the key protein involved in the early formation of egg-white thermal gel. , 2021, Food chemistry.

[4]  Hongbo Song,et al.  Structural and rheological characterization of pectin from passion fruit (Passiflora edulis f. flavicarpa) peel extracted by high-speed shearing , 2021, Food Hydrocolloids.

[5]  A. Handa,et al.  Role of lysozyme on liquid egg white foaming properties: Interface behavior, physicochemical characteristics and protein structure , 2021 .

[6]  F. Geng,et al.  Microwave pretreatment enhanced the properties of ovalbumin-inulin-oil emulsion gels and improved the storage stability of pomegranate seed oil , 2021 .

[7]  F. Geng,et al.  Depolymerization of chicken egg yolk granules induced by high-intensity ultrasound. , 2021, Food chemistry.

[8]  F. Saeed,et al.  Structural and functional properties of milk proteins as affected by heating, high pressure, Gamma and ultraviolet irradiation: a review , 2021, International Journal of Food Properties.

[9]  F. Geng,et al.  Tandem mass tag-labeled quantitative proteomic analysis of tenderloins between Tibetan and Yorkshire pigs. , 2020, Meat science.

[10]  F. Geng,et al.  Quantitative N-glycoproteomic analyses provide insights into the effects of thermal processes on egg white functional properties. , 2020, Food chemistry.

[11]  Z. Cai,et al.  Mechanism of enhancing foaming properties of egg white by super critical carbon dioxide treatment. , 2020, Food chemistry.

[12]  Xuebo Liu,et al.  Effects of ball-milling treatment on physicochemical and foaming activities of egg ovalbumin , 2019, Journal of Food Engineering.

[13]  Tian Ding,et al.  Preparation of modified whey protein isolate with gum acacia by ultrasound maillard reaction , 2019, Food Hydrocolloids.

[14]  Cuiping Han,et al.  Encapsulation of curcumin in zein/ caseinate/sodium alginate nanoparticles with improved physicochemical and controlled release properties , 2019, Food Hydrocolloids.

[15]  Tianqi Li,et al.  Impact of ultrasonic power on the structure and emulsifying properties of whey protein isolate under various pH conditions , 2019, Process Biochemistry.

[16]  Yujie Su,et al.  Emulsifying properties of glycation or glycation-heat modified egg white protein. , 2019, Food research international.

[17]  S. Yeganehzad,et al.  Effect of Persian gum and Xanthan gum on foaming properties and stability of pasteurized fresh egg white foam , 2019, Food Hydrocolloids.

[18]  A. Moosavi-Movahedi,et al.  Effect of free radical-induced aggregation on physicochemical and interface-related functionality of egg white protein , 2019, Food Hydrocolloids.

[19]  M. Ma,et al.  Effect of microwave-assisted phosphorylation modification on the structural and foaming properties of egg white powder , 2018, LWT.

[20]  F. Geng,et al.  Effect of hydroxyl radical‐induced oxidation on the structure and heat‐induced gel properties of ovalbumin , 2018 .

[21]  M. Ma,et al.  Influence of high-intensity ultrasound on foaming and structural properties of egg white. , 2018, Food research international.

[22]  M. Ma,et al.  A study of storage impact on ovalbumin structure of chicken egg , 2018 .

[23]  Jie Chen,et al.  Physicochemical and functional properties of protein extracts from Torreya grandis seeds. , 2017, Food chemistry.

[24]  Siyi Pan,et al.  Ball-milling changed the physicochemical properties of SPI and its cold-set gels , 2017 .

[25]  V. Fogliano,et al.  High-Pressure-High-Temperature Processing Reduces Maillard Reaction and Viscosity in Whey Protein-Sugar Solutions. , 2016, Journal of agricultural and food chemistry.

[26]  Yuan-ming Sun,et al.  Comparative studies on physicochemical properties of bovine serum albumin-glucose and bovine serum albumin-mannose conjugates formed via Maillard reaction , 2016 .

[27]  Yujie Su,et al.  Characteristics and emulsifying properties of acid and acid-heat induced egg white protein , 2016 .

[28]  Qian Liu,et al.  Structure and antioxidant activity of whey protein isolate conjugated with glucose via the Maillard reaction under dry-heating conditions , 2014 .

[29]  Jianhua Liu,et al.  Glycation a promising method for food protein modification: Physicochemical properties and structure, a review , 2012 .

[30]  Ke‐Xue Zhu,et al.  Effects of high hydrostatic pressure treatment on allergenicity and structural properties of soybean protein isolate for infant formula , 2012 .

[31]  T. Zhang,et al.  Mechanism of microwave-accelerated soy protein isolate–saccharide graft reactions , 2011 .

[32]  L. Vázquez-Moreno,et al.  Conjugates of bovine serum albumin with chitin oligosaccharides prepared through the Maillard reaction. , 2010, Journal of agricultural and food chemistry.

[33]  K. Umemura,et al.  Preparation and characterization of Maillard reacted chitosan films with hemicellulose model compounds , 2008 .

[34]  Timothy J. Mason,et al.  Effect of ultrasound treatment on solubility and foaming properties of whey protein suspensions , 2008 .

[35]  T. Wooster,et al.  Rheology of whey protein–dextran conjugate films at the air/water interface , 2007 .

[36]  I. V. D. Plancken,et al.  Foaming properties of egg white proteins affected by heat or high pressure treatment , 2007 .

[37]  M. E. Morales,et al.  An experimental investigation of the stability of ethylcellulose latex: correlation between zeta potential and sedimentation. , 2005, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[38]  G. P. Rizzi.  Role of phosphate and carboxylate ions in maillard browning. , 2004, Journal of agricultural and food chemistry.

[39]  M. V. van Boekel,et al.  Kinetic modeling of reactions in heated monosaccharide-casein systems. , 2002, Journal of agricultural and food chemistry.

[40]  A. Kato,et al.  Relationship between surface functional properties and flexibility of proteins detected by the protease susceptibility , 1985 .

[41]  Fengping An,et al.  Ball-milling is an effective pretreatment of glycosylation modified the foaming and gel properties of egg white protein , 2022, Journal of Food Engineering.

[42]  H. van den Brand,et al.  Egg storage and breeder age impact on egg quality and embryo development. , 2019, Journal of animal physiology and animal nutrition.

[43]  D. Żmudziński,et al.  The effect of pectins and xanthan gum on physicochemical properties of egg white protein foams , 2015 .