In vitro genetics.

Understanding the basis of specificity in an intermolecular interaction is a common if difficult task; designing a specific intermolecular interaction is much more challenging. A new technique is described that has applications to both problems, at least with regard to nucleic acids. The power of this method lies in its ability to isolate extremely rare sequences with precisely specified properties from very large pools of random sequences.

[1]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[2]  T. Cech,et al.  Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena , 1982, Cell.

[3]  H. Weintraub,et al.  Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection. , 1990, Science.

[4]  W. Kaelin,et al.  The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein , 1991, Cell.

[5]  D Botstein,et al.  Many random sequences functionally replace the secretion signal sequence of yeast invertase. , 1987, Science.

[6]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[7]  F. Kramer,et al.  Evolution in vitro: sequence and phenotype of a mutant RNA resistant to ethidium bromide. , 1974, Journal of molecular biology.

[8]  K. Kinzler,et al.  The GLI gene encodes a nuclear protein which binds specific sequences in the human genome , 1990, Molecular and cellular biology.

[9]  R. Gronostajski.  Site-specific DNA binding of nuclear factor I: effect of the spacer region. , 1987, Nucleic acids research.

[10]  Gerald F. Joyce,et al.  Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA , 1990, Nature.

[11]  Directed evolution of nucleic acids by independent replication and selection. , 1990, Science.

[12]  Michael R. Green,et al.  HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA , 1991, Cell.

[13]  H. Weintraub,et al.  Sequence-specific DNA binding by the c-Myc protein. , 1990, Science.

[14]  F. Kramer,et al.  Autocatalytic replication of a recombinant RNA. , 1983, Journal of molecular biology.

[15]  K. Kinzler,et al.  Whole genome PCR: application to the identification of sequences bound by gene regulatory proteins. , 1989, Nucleic acids research.

[16]  R. Treisman,et al.  A sensitive method for the determination of protein-DNA binding specificities. , 1990, Nucleic acids research.

[17]  R. Green,et al.  In vitro genetic analysis of the Tetrahymena self-splicing intron , 1990, Nature.

[18]  Jun Ma,et al.  A new class of yeast transcriptional activators , 1987, Cell.

[19]  N. Copeland,et al.  Evi-1, a murine zinc finger proto-oncogene, encodes a sequence-specific DNA-binding protein , 1991, Molecular and cellular biology.

[20]  K. Struhl,et al.  The use of random-sequence oligonucleotides for determining consensus sequences. , 1987, Methods in enzymology.

[21]  H. Thiesen,et al.  Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein. , 1990, Nucleic acids research.

[22]  L. Loeb,et al.  Mutants generated by the insertion of random oligonucleotides into the active site of the beta-lactamase gene. , 1989, Biochemistry.

[23]  Oligonucleotide-based therapeutics , 1991, Nature.