RuBi-Glutamate: Two-Photon and Visible-Light Photoactivation of Neurons and Dendritic spines

We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations, partly avoiding the blockade of GABAergic transmission present with other caged compounds. Two-photon uncaging of RuBi-Glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore enables the photoactivation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single cell, or even single spine, precision.

[1]  E. Callaway,et al.  Photostimulation using caged glutamate reveals functional circuitry in living brain slices. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[2]  K. Gee,et al.  Photolabile precursors of glutamate: synthesis, photochemical properties, and activation of glutamate receptors on a microsecond time scale. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Rafael Yuste,et al.  A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes. , 2010, Journal of inorganic biochemistry.

[4]  Karel Svoboda,et al.  Circuit Analysis of Experience-Dependent Plasticity in the Developing Rat Barrel Cortex , 2003, Neuron.

[5]  M Canepari,et al.  Photochemical and pharmacological evaluation of 7-nitroindolinyl-and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters , 2001, Journal of Neuroscience Methods.

[6]  Karel Svoboda,et al.  NMDA Receptor Subunit-Dependent [Ca2+] Signaling in Individual Hippocampal Dendritic Spines , 2005, The Journal of Neuroscience.

[7]  Rafael Yuste,et al.  Imaging in Neuroscience and Development: A Laboratory Manual , 2004 .

[8]  Rafael Yuste,et al.  Photorelease of GABA with Visible Light Using an Inorganic Caging Group , 2008, Frontiers in neural circuits.

[9]  G. Ellis‐Davies.  Basics of photoactivation. , 2009, Cold Spring Harbor Protocols.

[10]  J. Magee,et al.  State-Dependent Dendritic Computation in Hippocampal CA1 Pyramidal Neurons , 2006, The Journal of Neuroscience.

[11]  Rafael Yuste,et al.  Two-photon photostimulation and imaging of neural circuits , 2007, Nature Methods.

[12]  Roberto Araya,et al.  The spine neck filters membrane potentials , 2006, Proceedings of the National Academy of Sciences.

[13]  W Zieglgänsberger,et al.  Glutamate receptors form hot spots on apical dendrites of neocortical pyramidal neurons. , 2001, Journal of neurophysiology.

[14]  M A Xu-Friedman,et al.  Probing Fundamental Aspects of Synaptic Transmission with Strontium , 2000, The Journal of Neuroscience.

[15]  Karel Svoboda,et al.  Precise Development of Functional and Anatomical Columns in the Neocortex , 2004, Neuron.

[16]  G. Ellis‐Davies,et al.  Structural basis of long-term potentiation in single dendritic spines , 2004, Nature.

[17]  George J Augustine,et al.  Chemical Two-Photon Uncaging: a Novel Approach to Mapping Glutamate Receptors , 1997, Neuron.

[18]  松崎 政紀.  Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons , 2001 .

[19]  J. Nadler,et al.  γ-Aminobutyrate, α-carboxy-2-nitrobenzyl ester selectively blocks inhibitory synaptic transmission in rat dentate gyrus , 2000 .

[20]  B. Sabatini,et al.  State-Dependent Calcium Signaling in Dendritic Spines of Striatal Medium Spiny Neurons , 2004, Neuron.

[21]  Roberto Etchenique,et al.  A new strategy for neurochemical photodelivery: metal-ligand heterolytic cleavage. , 2003, Journal of the American Chemical Society.

[22]  Brendon O. Watson,et al.  SLM Microscopy: Scanless Two-Photon Imaging and Photostimulation with Spatial Light Modulators , 2008, Frontiers in neural circuits.

[23]  Rafael Yuste,et al.  Stimulating neurons with light , 2002, Current Opinion in Neurobiology.

[24]  E. Callaway,et al.  Excitatory cortical neurons form fine-scale functional networks , 2005, Nature.

[25]  J. Corrie,et al.  Comparative analysis of inhibitory effects of caged ligands for the NMDA receptor , 2005, Journal of Neuroscience Methods.