Development of a matrix solid-phase dispersion method for the screening of polybrominated diphenyl ethers and polychlorinated biphenyls in biota samples using gas chromatography with electron-capture detection.

A low cost method for the screening of six polybrominated diphenyl ethers (PBDEs) and seven polychlorinated biphenyls (PCBs) in biological samples containing up to 100% of fat is presented. Compounds are extracted from the sample and isolated from lipids using a matrix solid-phase dispersion (MSPD) cartridge and 20 ml of n-hexane as elution solvent. PBDEs and PCBs are fractionated on a second cartridge containing 2 g of a normal phase sorbent. The potential of neutral silica, Florisil and basic alumina to separate PBDEs and PCBs in two independent fractions has been evaluated. The best results were obtained using silica. PCBs are eluted, in a first fraction, using n-hexane. PBDEs are further recovered with n-hexane-dichloromethane. The applicability of the method for the screening of PBDEs and PCBs, in samples containing both groups of compounds, has been demonstrated using spiked, certified and real polluted samples from different biota materials. Globally, recoveries higher than 75% and quantification limits around 0.4 ng/g have been achieved using gas chromatography with electron-capture detection (GC-ECD).

[1]  Sierra Rayne,et al.  Rapidly increasing polybrominated diphenyl ether concentrations in the Columbia River system from 1992 to 2000. , 2003, Environmental science & technology.

[2]  P. Saukko,et al.  Identification and quantification of polychlorinated biphenyls and some endocrine disrupting pesticides in human adipose tissue from Finland. , 2001, Chemosphere.

[3]  C. Thomsen,et al.  A new method for determination of halogenated flame retardants in human milk using solid-phase extraction. , 2002, Journal of analytical toxicology.

[4]  A. Covaci,et al.  Determination of polybrominated diphenyl ethers and polychlorinated biphenyls in human adipose tissue by large-volume injection-narrow-bore capillary gas chromatography/electron impact low-resolution mass spectrometry. , 2002, Analytical chemistry.

[5]  Mitchell D. Erickson,et al.  Analytical Chemistry of PCBs , 1986 .

[6]  B. Jansson,et al.  Multiresidue method for the gas-chromatographic analysis of some polychlorinated and polybrominated pollutants in biological samples , 1991 .

[7]  S. Bayen,et al.  Determination of polybrominated diphenyl ethers in marine biological tissues using microwave-assisted extraction. , 2004, Journal of chromatography. A.

[8]  J. Gómez-Ariza,et al.  Determination of polychlorinated biphenyls in biota samples using simultaneous pressurized liquid extraction and purification. , 2002, Journal of chromatography. A.

[9]  R. C. Torrijos,et al.  Application of matrix solid-phase dispersion to the determination of polychlorinated biphenyls in fat by gas chromatography with electron-capture and mass spectrometric detection. , 2004 .

[10]  R. Law,et al.  Method for the analysis of polybrominated diphenylethers in sediments and biota , 2001 .

[11]  W. Sonzogni,et al.  Comparison of polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) in Lake Michigan salmonids. , 2001, Environmental science & technology.

[12]  Ronald A Hites,et al.  Polybrominated diphenyl ethers in the environment and in people: a meta-analysis of concentrations. , 2004, Environmental science & technology.

[13]  R. Cela,et al.  Development of a solid-phase microextraction gas chromatography/tandem mass spectrometry method for polybrominated diphenyl ethers and polybrominated biphenyls in water samples. , 2004, Analytical chemistry.

[14]  S. Sakai,et al.  Modified clean-up for PBDD, PBDF and PBDE with an active carbon column--its application to sediments. , 2003, Chemosphere.

[15]  Robert C Hale,et al.  Potential role of fire retardant-treated polyurethane foam as a source of brominated diphenyl ethers to the US environment. , 2002, Chemosphere.

[16]  G. O. Thomas,et al.  Occurrence of polychlorinated biphenyls and polybrominated diphenyl ethers in green mussels (Perna viridis) from Singapore, Southeast Asia , 2003, Environmental toxicology and chemistry.

[17]  D. Barceló,et al.  Determination of 39 polybrominated diphenyl ether congeners in sediment samples using fast selective pressurized liquid extraction and purification. , 2003, Journal of chromatography. A.

[18]  E. De Pauw,et al.  New strategy for comprehensive analysis of polybrominated diphenyl ethers, polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and polychlorinated biphenyls by gas chromatography coupled with mass spectrometry. , 2003, Journal of chromatography. A.

[19]  C. D. de Wit,et al.  Higher brominated diphenyl ethers and hexabromocyclododecane found in eggs of peregrine falcons (Falco peregrinus) breeding in Sweden. , 2004, Environmental science & technology.

[20]  Peter Haglund,et al.  Identification and Quantification of Polybrominated Diphenyl Ethers and Methoxy-Polybrominated Diphenyl Ethers in Baltic Biota , 1997 .

[21]  C. Östman,et al.  Retention characteristics of some selected halogenated environmental pollutants in silica and bonded normal-phase liquid chromatography , 1994 .

[22]  M. Hardy.  A comparison of the properties of the major commercial PBDPO/PBDE product to those of major PBB and PCB products. , 2002, Chemosphere.

[23]  R C Hale,et al.  Polybrominated diphenyl ether flame retardants in Virginia freshwater fishes (USA). , 2001, Environmental science & technology.

[24]  Adrian Covaci,et al.  Investigation of selected persistent organic pollutants in farmed Atlantic salmon (Salmo salar), salmon aquaculture feed, and fish oil components of the feed. , 2002, Environmental science & technology.

[25]  G. Blomkvist,et al.  Polybrominated aromatic pollutants found in fish in Sweden , 1981 .

[26]  J. Boer.  Organochlorine compounds and bromodiphenylethers in livers of Atlantic cod (Gadus morhua) from the North Sea, 1977-1987 , 1989 .

[27]  Christian Lydersen,et al.  Congener-specific accumulation and food chain transfer of polybrominated diphenyl ethers in two arctic food chains. , 2004, Environmental science & technology.