Dynamic DNA Damage and Repair Modeling: Bridging the Gap Between Experimental Damage Readout and Model Structure

In this work, a method is presented to map a set of experimentally obtained, time-resolved distributions to a dynamic model. Specifically, time-resolved comet assay readouts of cancer cells after application of ionizing radiation are mapped to the Multi-Hit-Repair model, a radiobiologically motivated dynamic model used to predict DNA damage and repair. Differential evolution is used for parameter-search to showcase the potential of this method, producing a prediction close to the experimental measurement. The results obtained from the parameter search are used to characterize aspects of the repair process. The method is compared to prior attempts of finding model parameters from dose-response curves, revealing that calibration is required to render the two comparable.

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