Angiotensin converting enzyme inhibitors attenuated the expression of G-protein coupled receptor kinases in heart failure patients.

BACKGROUND There are few biological markers, which strictly show the severity of congestive heart failure (CHF). METHODS AND RESULTS Lymphocyte G-protein coupled receptor kinase (GRK) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction in 15 CHF patients: 5 patients classified as New York Heart Association class-II treated with angiotensin converting enzyme inhibitor (ACEI) (IIA), 5 patients in class-II without ACEI (IIC), and 5 patients in class-III treated with ACEI (IIIA). GRK mRNA level in IIIA was significantly higher than those in IIA (p<0.05). GRK mRNA level in IIA were significantly lower than those in IIC (p<0.05). CONCLUSIONS The expression level of lymphocyte GRK might show the severity of CHF, and ACEI treatment could reduce the level of GRK in CHF patients.

[1]  K. Urasawa,et al.  Chronic beta-adrenergic receptor stimulation enhances the expression of G-Protein coupled receptor kinases, GRK2 and GRK5, in both the heart and peripheral lymphocytes. , 2005, Circulation journal : official journal of the Japanese Circulation Society.

[2]  J. Bijlsma,et al.  Decreased expression and activity of G‐protein‐coupled receptor kinases in peripheral blood mononuclear cells of patients with rheumatoid arthritis , 1999, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[3]  M. Sallese,et al.  G protein-coupled receptors: heterologous regulation of homologous desensitization and its implications. , 1996, Trends in pharmacological sciences.

[4]  K. Urasawa,et al.  Enhanced expression of beta-adrenergic receptor kinase 1 in the hearts of cardiomyopathic Syrian hamsters, BIO53.58. , 1996, Biochemical and biophysical research communications.

[5]  K. Kameyama,et al.  G Protein‐Coupled Receptor Kinases , 1994 .

[6]  J. Benovic,et al.  Structure of the human gene encoding the beta-adrenergic receptor kinase. , 1994, The Journal of biological chemistry.

[7]  M. Böhm,et al.  Altered expression of beta-adrenergic receptor kinase and beta 1-adrenergic receptors in the failing human heart. , 1993, Circulation.

[8]  K. Urasawa,et al.  A mechanism of catecholamine tolerance in congestive heart failure--alterations in the hormone sensitive adenylyl cyclase system of the heart. , 1992, Japanese circulation journal.

[9]  M. Caron,et al.  beta-Arrestin: a protein that regulates beta-adrenergic receptor function. , 1990, Science.

[10]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[11]  高木 千佳.  Enhanced GRK5 Expression in the Hearts of Cardiomyopathic Hamsters,J2N-k , 2001 .

[12]  A. Böyum,et al.  Isolation of leucocytes from human blood. A two-phase system for removal of red cells with methylcellulose as erythrocyte-aggregating agent. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.