Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G3), which was generated from the diploid eggs produced by the second gynogenetic generation (G2) of red crucian carp × common carp, and determined the chromosomal numbers of G3, G2×scatter scale carp and G2×allotetraploid hybrids of red crucian carp × common carp. The results showed that the diploid eggs of G2 with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G3 with 100 chromosomes. Similar to the first and second gynogenetic generations (G1 and G2), G3 was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G2 with scatter scale carp, and crossing G2 with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G2 ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G2. The establishment of the diploid gynogenesis clonal line (G1, G2 and G3) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gynogenetic progeny of allotetraploid hybrids of red crucian carp × common carp had important significances in both biological evolution and production application.

[1]  Ning Li,et al.  Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer , 2006, Science in China Series C: Life Sciences.

[2]  C. Chunpeng,et al.  Hydrogen peroxide homeostasis and signaling in plant cells. , 2006 .

[3]  H. Feng,et al.  The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization , 2001 .

[4]  Shaojun Liu,et al.  RAPD and microsatellite analysis of diploid gynogens from allotetraploid hybrids of red crucian carp (Carassius auratus) common carp (Cyprinus carpio) , 2005 .

[5]  Shao J Liu,et al.  [Evidence of the unreduced diploid eggs generated from the diploid gynogenetic progeny of allotetraploid hybrids]. , 2005, Yi chuan xue bao = Acta genetica Sinica.

[6]  Shaojun Liu,et al.  Evidence for Recombination of Mitochondrial DNA in Triploid Crucian Carp , 2006, Genetics.

[7]  Shaojun Liu,et al.  Comparative and Evolutionary Analysis of the Cytochrome b Sequences in Cyprinids with Different Ploidy Levels Derived from Crosses , 2004, Genetica.

[8]  Yuzhen Ye,et al.  Two unisexual artificial polyploid clones constructed by genome addition of common carp (Cyprinus carp) and crucian carp (Carassius auratus) , 2003, Science in China Series C: Life Sciences.

[9]  Shaojun Liu,et al.  Comparative analysis of the mitochondrial DNA control region in cyprinids with different ploidy level , 2003 .

[10]  Liu Shaojun,et al.  Comparative studies on histological and ultra-structure of the pituitary of different ploidy level fishes. , 2006 .

[11]  Shao J Liu,et al.  [The chromosome number and gonadal structure of F9-F11 allotetraploid crucian-carp]. , 2003, Yi chuan xue bao = Acta genetica Sinica.

[12]  O. Emelyanova,et al.  Induced diploid gynogenesis and polyploidy in crucian carp, Carassius auratus gibelio (Bloch), x common carp, Cyprinus carpio L., hybrids. , 1994 .

[13]  G. Hulata.  Genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies , 2004, Genetica.

[14]  T. Pandian,et al.  Ploidy induction and sex control in fish , 1998, Hydrobiologia.

[15]  Shaojun Liu,et al.  Production of gynogenetic progeny from allotetraploid hybrids red crucian carp×common carp , 2004 .