Assessment of a DNA vaccine encoding an anchored-glycosylphosphatidylinositol tegumental antigen complexed to protamine sulphate on immunoprotection against murine schistosomiasis.

Protamine sulphate/DNA complexes have been shown to protect DNA from DNase digestion in a lipid system for gene transfer. A DNA-based vaccine complexed to protamine sulphate was used to induce an immune response against Schistosoma mansoni anchored-glycosylphosphatidylinositol tegumental antigen in BALB/c mice. The protection elicited ranged from 33 to 44%. The spectrum of the elicited immune response induced by the vaccine formulation without protamine was characterized by a high level of IgG (IgG1> IgG2a). Protamine sulphate added to the DNA vaccine formulation retained the green fluorescent protein encoding-plasmid longer in muscle and spleen. The experiments in vivo showed that under protamine sulphate effect, the scope of protection remained unchanged, but a modulation in antibody production (IgG1= IgG2a) was observed.

[1]  G. Cassali,et al.  Co-administration of plasmid expressing IL-12 with 14-kDa Schistosoma mansoni fatty acid-binding protein cDNA alters immune response profiles and fails to enhance protection induced by Sm14 DNA vaccine alone. , 2006, Microbes and infection.

[2]  D. Harn,et al.  Schistosoma japonicum triose-phosphate isomerase plasmid DNA vaccine protects pigs against challenge infection , 2005, Parasitology.

[3]  D. McManus.  Prospects for development of a transmission blocking vaccine against Schistosoma japonicum , 2005, Parasite immunology.

[4]  L. Leonardo,et al.  Vaccine-linked chemotherapy: can schistosomiasis control benefit from an integrated approach? , 2005, Trends in parasitology.

[5]  A. Siddiqui,et al.  Characterization of protective immunity induced against Schistosoma mansoni via DNA priming with the large subunit of calpain (Sm-p80) in the presence of genetic adjuvants. , 2005, Parasite.

[6]  P. LoVerde,et al.  Protection against Schistosoma mansoni utilizing DNA vaccination with genes encoding Cu/Zn cytosolic superoxide dismutase, signal peptide-containing superoxide dismutase and glutathione peroxidase enzymes. , 2003, Vaccine.

[7]  R. Podesta,et al.  Induction of Protective Immunity against Schistosoma mansoni via DNA Priming and Boosting with the Large Subunit of Calpain (Sm-p80): Adjuvant Effects of Granulocyte-Macrophage Colony-Stimulating Factor and Interleukin-4 , 2003, Infection and Immunity.

[8]  R. Podesta,et al.  Enhancement of Sm-p80 (large subunit of calpain) induced protective immunity against Schistosoma mansoni through co-delivery of interleukin-2 and interleukin-12 in a DNA vaccine formulation. , 2003, Vaccine.

[9]  E. Pearce.  Progress towards a vaccine for schistosomiasis. , 2003, Acta tropica.

[10]  I. C. Leão,et al.  Protective immunity of single and multi-antigen DNA vaccines against schistosomiasis. , 2002, Memorias do Instituto Oswaldo Cruz.

[11]  S. Visovatti,et al.  Comparative efficacy of the Schistosoma mansoni nucleic acid vaccine, Sm23, following microseeding or gene gun delivery , 2002, Parasite immunology.

[12]  A. Da'dara,et al.  Immunization with plasmid DNA encoding the integral membrane protein, Sm23, elicits a protective immune response against schistosome infection in mice. , 2001, Vaccine.

[13]  A. Thomas,et al.  Cellular and Humoral Immune Responses and Protection against Schistosomes Induced by a Radiation-Attenuated Vaccine in Chimpanzees , 2001, Infection and Immunity.

[14]  Y. Rojanasakul,et al.  High-efficiency gene transfection of macrophages by lipoplexes. , 2000, International journal of pharmaceutics.

[15]  A. Riis,et al.  Pharmacokinetics and pharmacodynamics of a premixed formulation of soluble and protamine-retarded insulin aspart , 2000, European Journal of Clinical Pharmacology.

[16]  K. Porter,et al.  Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques. , 2000, The Journal of general virology.

[17]  J. Birchall,et al.  Physical stability and in-vitro gene expression efficiency of nebulised lipid-peptide-DNA complexes. , 2000, International journal of pharmaceutics.

[18]  R. Repp,et al.  Nuclear gene targeting using negatively charged liposomes. , 2000, International journal of pharmaceutics.

[19]  H. Robinson.  DNA vaccines: basic mechanism and immune responses (Review). , 1999, International journal of molecular medicine.

[20]  M. Chang,et al.  Use of protamine to augment adenovirus-mediated cancer gene therapy , 1999, Gene Therapy.

[21]  M. Kamihira,et al.  Enhancement of transfection efficiency by protamine in DDAB lipid vesicle-mediated gene transfer. , 1999, Journal of biochemistry.

[22]  R. Podesta,et al.  Recombinant vaccinia viruses and gene gun vectors expressing the large subunit of Schistosoma mansoni calpain used in a murine immunization-challenge model. , 1999, Vaccine.

[23]  Mcmanus,et al.  DNA immunization by intramuscular injection or gene gun induces specific IgG antibodies against a Schistosoma japonicum 22 kDa antigen, Sj22, when fused to the murine Ig K‐chain secretory leader sequence , 1999, Parasite immunology (Print).

[24]  A. Sher,et al.  Optimal vaccination against Schistosoma mansoni requires the induction of both B cell- and IFN-gamma-dependent effector mechanisms. , 1999, Journal of immunology.

[25]  D. Weiner,et al.  Modulating the immune response to genetic immunization , 1998, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[26]  Leaf Huang,et al.  Potentiation of cationic liposome-mediated gene delivery by polycations. , 1996, Biochemistry.

[27]  D. McManus,et al.  Antibodies to Schistosoma japonicum (Asian bloodfluke) paramyosin induced by nucleic acid vaccination. , 1995, Biochemical and biophysical research communications.

[28]  M. Strand,et al.  Selective release of a glycosylphosphatidylinositol-anchored antigen from the surface of Schistosoma mansoni. , 1991, Molecular and biochemical parasitology.

[29]  A. Sher,et al.  Downregulation of Th1 cytokine production accompanies induction of Th2 responses by a parasitic helminth, Schistosoma mansoni , 1991, The Journal of experimental medicine.

[30]  A. Sher,et al.  Role of host antibody in the chemotherapeutic action of praziquantel against Schistosoma mansoni: identification of target antigens. , 1989, Molecular and biochemical parasitology.

[31]  Z. Li,et al.  Nephelometric determination of micro amounts of nucleic acids with protamine sulfate. , 1999, The Analyst.

[32]  L. Huang,et al.  Protamine sulfate enhances lipid-mediated gene transfer , 1997, Gene Therapy.