Variability in the cardiometabolic effects of ω-3 long-chain PUFAs: background diet, timing, and genetics.

Omega-3 long-chain PUFAs (LC-PUFAs) have received considerable attention over many years for their proposed health benefits, particularly in relation to cardiovascular disease. More recently, there has been interest in the potential for x-3 LC-PUFAs to favorably influence other metabolic variables, including inhibiting weight gain during exposure to “obesogenic” diets and aiding in the loss of body weight and body fat. Despite some encouraging data from in vitro and animal studies and a plausible biological basis for the favorable effects of x-3 LC-PUFAs on these outcomes, the results from human studies have been less than conclusive. Current meta-analyses in the cardiovascular field, for example, conclude that there is no strong evidence that dietary or supplemental x-3 fats alter the risk of combined cardiovascular events, either in individuals at high risk or in the general population (1). The conclusions of the most recent systematic review of studies focused on x-3 LC-PUFAs and body composition are somewhat more positive and indicate that fish or fish-oil supplementation produces greater losses in body weight, BMI, and waist circumference in comparison to placebo (2); however, it is important to note the much lower number of studies on this topic and that supplementation is typically applied on top of reduced overall energy intake. A feature of thex-3 fatty acid literature, as with many nutritional fields, is the variability in the effects reported in different studies. Part of this inconsistency is likely to be due to differences in study design (e.g., dosage and timing of intervention) and the background x-3 status of the population. It is also clear, however, that the effects of x-3 LC-PUFA intake and supplementation can vary substantially between individuals, a phenomenon that appears to be at least in part a result of interindividual differences in the relation between fatty acid intakes and the concentrations of their downstream bioactive mediators (3). The implication of this is that conclusions based on studies in the general population, or in one ethnic group, may be limited in informing whether there are particular subgroups who are more likely to benefit. This issue of the Journal features 3 new studies that investigate the relation between x-3 intake and status and cardiovascular and body weight outcomes across 3 different countries (Denmark, Singapore, and Costa Rica). The findings provide further indications of the complex interrelation between x-3 LC-PUFA intakes, x-3 LC-PUFA status, background diet, timing, and genetics and cardiometabolic variables. In the first study, Jakobsen et al. (4) investigate the relation between dietary intake and adipose tissue content of x-3 LC-PUFAs and 5-y changes in body weight and waist circumference in Danish adults. Despite some interesting trends, they found no consistent or appreciable association between habitual x-3 LC-PUFA intake or status at baseline and weight or waist circumference changes over the subsequent 5-y period. The findings align with those of the most recent systemic review, which suggested that although short-term periods of supplementation (,2 mo) could favorably influenceweight loss, there was no evidence of associations with longer-term intakes (2). The study by Jakobsen et al. also found no evidence to suggest that the effects of x-3 LC-PUFAs on weight or waist circumference are dependent on the carbohydrate-to-protein ratio or glycemic index of the background diet, a finding reported in previous animal studies (5, 6). This is perhaps not surprising when one examines the composition of the diets used in the animal studies, which encompassed much greater extremes of both x-3 and macronutrient intakes than in this human study. This finding thus highlights the inherent difficulty in extrapolating results of dietary studies in animals to the range of nutritional intakes commonly encountered in human diets. In contrast to these findings, the study by Loy et al. (7), also featured in this issue of the Journal, reports that higher x-3 LC-PUFA intakes or status at 26–28 wk of pregnancy were associated with lower weight retention at 18 mo postpartum in Singaporean women. Although it is not possible to assess causality in either of these observational studies, and there is the potential of residual confounding, they nevertheless raise the possibility that any effects of x-3 LC-PUFAs on body weight may be dependent on the timing of intervention, and perhaps the physiologic status of the individual (i.e., whether they are in positive or negative energy balance). Genetic factors have also been shown to influence the response of individuals to dietary x-3 supplementation. Much of this work to date has focused on the impact of single nucleotide polymorphisms