Epidermal Langerhans cells--a target for HTLV-III/LAV infection.

Langerhans cells (LC) are bone marrow-derived, Ia+, CD1+, CD4+, ATPase+ dendritic antigen-presenting cells within the human epidermis. Since the CD4 molecule has been implicated as a receptor structure for HTLV-III/LAV (human T-cell leukemia virus/lymphadenopathy-associated virus), we asked whether LC from HTLV-III/LAV-seropositive individuals display signs of HTLV-III/LAV infection. In skin biopsies from 7/40 HTLV-III/LAV-infected persons (1 asymptomatic carrier, 2 patients with acquired immunodeficiency syndrome (AIDS)-related complex and 4 patients with AIDS), LC were the only epidermal cells to react with a monoclonal antibody specific for the HTLV-III core protein p17. A varying percentage of p17+ LC were morphologically altered with blunt dendrites and poorly demarcated cellular contours. In one of these biopsies, the presence of LC-associated viral particles characteristic of HTLV-III/LAV as well as cytopathic changes in approximately one-third of the LC population were demonstrated by electron microscopy. These results strongly suggest that LC may harbor HTLV-III/LAV. The infection of LC with this retrovirus may have deleterious consequences for the immunologic functions of this cell system and may thus contribute to both the acquisition of immunodeficiency and the infectious and neoplastic complications of AIDS.

[1]  D. Markovitz,et al.  Virus isolation from and identification of HTLV-III/LAV-producing cells in brain tissue from a patient with AIDS. , 1986, JAMA.

[2]  D. Markovitz,et al.  The role of mononuclear phagocytes in HTLV-III/LAV infection. , 1986, Science.

[3]  J. Mcdougal,et al.  In vitro infection of human monocytes with human T lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV). , 1986, Journal of immunology.

[4]  M. Hirsch,et al.  Infection of monocyte/macrophages by human T lymphotropic virus type III. , 1986, The Journal of clinical investigation.

[5]  M. Dietrich,et al.  HTLV-III/LAV viral antigens in lymph nodes of homosexual men with persistent generalized lymphadenopathy and AIDS. , 1986, The American journal of pathology.

[6]  R. Gallo,et al.  Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[7]  K. Wolff,et al.  Leu-3/T4 expression on epidermal Langerhans cells in normal and diseased skin. , 1986, The Journal of investigative dermatology.

[8]  J K Nicholson,et al.  Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule. , 1986, Science.

[9]  R. Gallo,et al.  Human T-lymphotropic retroviruses , 1985, Nature.

[10]  R. Warnke,et al.  The immunohistology of non-T cells in the acquired immunodeficiency syndrome. , 1985, The American journal of pathology.

[11]  R. Gallo,et al.  Monoclonal antibodies specific for p24, the major core protein of human T-cell leukemia virus type III. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[12]  J. Armstrong,et al.  Retroviral infection of accessory cells and the immunological paradox in AIDS. , 1985, Immunology today.

[13]  R. Redfield,et al.  HTLV-III IN SYMPTOM-FREE SERONEGATIVE PERSONS , 1984, The Lancet.

[14]  Thomas Pa,et al.  AIDS outcome: a first follow-up. , 1984 .

[15]  C. Griscelli,et al.  Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes. , 1984, Science.

[16]  G. Thorbecke,et al.  Reduced Langerhans' cell Ia antigen and ATPase activity in patients with the acquired immunodeficiency syndrome. , 1984, The New England journal of medicine.

[17]  B. Haynes,et al.  Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS. , 1984, Science.

[18]  K. Wolff,et al.  Cytogenetic identification of allogeneic epidermal Langerhans cells in a bone-marrow-graft recipient. , 1984 .

[19]  R. Warnke,et al.  Anti-Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. , 1983, Journal of immunology.

[20]  K. Wolff,et al.  The langerhans cell. , 1983, The Journal of investigative dermatology.

[21]  J. Chermann,et al.  Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). , 1983, Science.

[22]  B. Haynes,et al.  Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny , 1983, The Journal of experimental medicine.

[23]  W. Shelley,et al.  New staining techniques for the Langerhans cell. , 1977, Acta dermato-venereologica.

[24]  Luc Montagnier,et al.  T-lymphocyte T4 molecule behaves as the receptor for human retrovirus  LAV , 1984, Nature.

[25]  M. Greaves,et al.  The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus , 1984, Nature.