Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai--Tibet Plateau permafrost region.

A psychrotolerant bacterium, designated strain Tibet-IIU11T, was isolated from the Qinghai--Tibet Plateau permafrost region, China. A taxonomic study was conducted using a polyphasic approach, with determination of physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences. The novel isolate was found to belong to the genus Hymenobacter and was distinct from the recognized species of this genus. The major fatty acids were iso-C15 : 0 (24.5 %), summed feature 3 (C16 : 1omega7c and/or iso C15 : 0 2-OH; 20.0 %), summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B; 14.0 %), C16 : 1omega5c (12.7 %) and anteiso-C15 : 0 (8.4 %). Phosphatidylethanolamine and an unknown aminophospholipid were predominant in the polar lipid profile. The quinone system consisted exclusively of menaquinone MK-7 and sym-homospermidine was the major polyamine present. These chemotaxonomic traits are in good agreement with the characteristics of the genus Hymenobacter. The assignment of the novel isolate to this genus was further supported by a DNA G+C content of 60 mol%. On the basis of the polyphasic evidence presented in this study, it is proposed that strain Tibet-IIU11T (=DSM 18569T=CGMCC 1.6365T) represents a novel species, Hymenobacter psychrotolerans sp. nov.

[1]  G. Cheng,et al.  Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region. , 2007, Canadian journal of microbiology.

[2]  A. Yokota,et al.  Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. , 2007, International journal of systematic and evolutionary microbiology.

[3]  A. Stolz,et al.  Pseudomonas knackmussii sp. nov. , 2007, International journal of systematic and evolutionary microbiology.

[4]  G. Cheng,et al.  Diversity and distribution of alkaliphilic psychrotolerant bacteria in the Qinghai–Tibet Plateau permafrost region , 2007, Extremophiles.

[5]  J. Chun,et al.  Hymenobacter rigui sp. nov., isolated from wetland freshwater. , 2006, International journal of systematic and evolutionary microbiology.

[6]  P. Kämpfer,et al.  Proposal of Hymenobacter norwichensis sp. nov., classification of 'Taxeobacter ocellatus', 'Taxeobacter gelupurpurascens' and 'Taxeobacter chitinovorans' as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended desc , 2006, International journal of systematic and evolutionary microbiology.

[7]  Michael P. Cummings,et al.  MEGA (Molecular Evolutionary Genetics Analysis) , 2004 .

[8]  P. Kämpfer,et al.  Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. , 2002, International journal of systematic and evolutionary microbiology.

[9]  Sudhir Kumar,et al.  MEGA2: molecular evolutionary genetics analysis software , 2001, Bioinform..

[10]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[11]  M. Collins,et al.  Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov. , 2000, International journal of systematic and evolutionary microbiology.

[12]  W. Ludwig,et al.  Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antartica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. , 1998, Systematic and applied microbiology.

[13]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[14]  Petra Altenburgera,et al.  Classification of bacteria isolated from a medieval wall painting , 1996 .

[15]  Erko Stackebrandt,et al.  Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology , 1994 .

[16]  E. Sherr,et al.  Effect of Protistan Grazing on the Frequency of Dividing Cells in Bacterioplankton Assemblages , 1992, Applied and environmental microbiology.

[17]  P. Kämpfer,et al.  Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria , 1991, Microbial Ecology.

[18]  B. Tindall.  A comparative study of the lipid composition of Halobacterium saccharovorum from various sources , 1990 .

[19]  G. Auling,et al.  Polyamine Pattern as a Chemotaxonomic Marker within the Proteobacteria , 1988 .

[20]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[21]  K. Moaledj.  Comparison of Gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy , 1986 .

[22]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[23]  K. Porter,et al.  The use of DAPI for identifying and counting aquatic microflora1 , 1980 .

[24]  J. T. Staley.  Prosthecomicrobium and Ancalomicrobium: New Prosthecate Freshwater Bacteria , 1968, Journal of bacteriology.

[25]  P. Doty,et al.  Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. , 1962, Journal of molecular biology.

[26]  James R. Cole,et al.  The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy , 2003, Nucleic Acids Res..

[27]  Hugh M. French,et al.  Permafrost on the Tibet Plateau, China , 1995 .

[28]  B. Tindall.  Lipid composition of Halobacterium lacusprofundi , 1990 .

[29]  W. Moore,et al.  Taxonomic Note : A Place for DNA-DNA Reassociation and 16 s rRNA Sequence Analysis in the Present Species Definition in , 2022 .