Bioreactors for Mammalian Cells

Cultivation of mammalian cells has become a routine technique. Nevertheless, design and layout of cell culture processes has to meet cell specific demands. Numerous bioreactors and cultivation systems for cell culture, either for production of biopharmaceuticals or for tissue engineering, have been developed. Due to the special characteristics of these cells specific solutions are required. Selection of a suitable type of cell culture bioreactor system and/or an appropriate operation mode (batch, fed-batch, and perfusion) is affected by technical, biological, economical and regulatory considerations. Obviously there is no single cultivation system suitable for all applications. Furthermore, to compare different bioreactor systems or operation modes experimentally for a certain application is time consuming and expensive. Therefore, selection of an appropriate bioreactor or cultivation system requires extensive knowledge and expertise. The following contribution gives an introduction to bioreactor systems and cultivation strategies applied.

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