Interactive control of holographic optical traps with fast hologram generation

Holographic optical tweezers (HOTs) make use of computer generated holograms to manipulate multiple optical traps. Uptill now, various algorithms have been proposed to calculate the hologram patterns. However the update rate of such holograms for a spatial light modulator (SLM) is often restricted to around 10Hz due to the slow computation in computer, limiting fast manipulation of optical traps in real-time. In this paper, we present the method and control software for interactive control of holographic optical traps with fast hologram generation by increasing the update rate of holograms. The developed software is written in Visual C++ platform and is capable of computing complex holograms with an update rate of 55 Hz, regardless of the number of traps. For experimental demonstrations, we show three dimensional, real-time manipulations of 5 µm polystyrene beads.

[1]  M J Padgett,et al.  The effect of external forces on discrete motion within holographic optical tweezers. , 2007, Optics express.

[2]  David McGloin,et al.  Optical tweezers: 20 years on , 2006, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[3]  Yael Roichman,et al.  Holographic optical trapping. , 2006, Applied optics.

[4]  Christoph F. Schmidt,et al.  Calibrating bead displacements in optical tweezers using acousto-optic deflectors , 2006 .

[5]  Jordi Andilla,et al.  Fast generation of holographic optical tweezers by random mask encoding of Fourier components. , 2006, Optics express.

[6]  R. Gerchberg.  A practical algorithm for the determination of phase from image and diffraction plane pictures , 1972 .

[7]  Johannes Courtial,et al.  Interactive approach to optical tweezers control. , 2006, Applied optics.

[8]  K. Svoboda,et al.  Biological applications of optical forces. , 1994, Annual review of biophysics and biomolecular structure.

[9]  David W. M. Marr,et al.  Design of a scanning laser optical trap for multiparticle manipulation , 2000 .

[10]  H. Misawa,et al.  Pattern formation and flow control of fine particles by laser-scanning micromanipulation. , 1991, Optics letters.

[11]  Joshua W Shaevitz,et al.  An automated two-dimensional optical force clamp for single molecule studies. , 2002, Biophysical journal.

[12]  Wolfgang Osten,et al.  Fast digital hologram generation and adaptive force measurement in liquid-crystal-display-based holographic tweezers. , 2006, Applied optics.

[13]  Min Wu,et al.  Using Graphics Boards to Compute Holograms , 2006, Comput. Sci. Eng..

[14]  Jennifer E. Curtis,et al.  Dynamic holographic optical tweezers , 2002 .

[15]  G. Spalding,et al.  Computer-generated holographic optical tweezer arrays , 2000, cond-mat/0008414.

[16]  David G. Grier,et al.  Optical tweezers in colloid and interface science , 1997 .

[17]  Naoya Matsumoto,et al.  Universal generation of higher-order multiringed Laguerre-Gaussian beams by using a spatial light modulator. , 2007, Optics letters.

[18]  H J Tiziani,et al.  Optical particle trapping with computer-generated holograms written on a liquid-crystal display. , 1999, Optics letters.