Factors influencing quality of palm oil produced at the cottage industry level in Ghana.

Palm oil is one of the major fats and oils produced and traded in the world today. It forms an important ingredient in the diet of many people in West Africa. The quality of palm oil is mostly determined by the fatty acid, moisture and impurity content, and bleachability. This study examined the quality of palm oil produced at cottage industry level in Ghana with a view to improve its quality to meet industrial standards and increase its marketability. Palm oil produced from fruits stored at various periods with various levels of contamination were examined over a twelve-month storage period for their microbial, fatty acid, moisture and impurity contents. PCR amplifications, sequencing and fragment analysis methods were used to identify microbes, while fatty acid (FFA), moisture and impurity indices were determined by titration and oven-dry methods. The major factors determining the fatty acid content was age of fruits used to process the oil and the length of storage of the oil after processing. The results show that good grade oils with low fatty acid (FFA) contain microbes, some of which have the potential of producing toxin. There was a direct relationship between storage period of oil and fatty acid content, and microbial loads in oils. As storage period increased, fatty acid and microbial load increased (r = 0.972 and 0.967). Lipolytic organisms identified are Aspergillus (0.27), Bacillus (0.08), Candida (1.34), Geotrichum (1.00) and Pseudomonas (2.11) species.

[1]  C. Airede,et al.  Deterioration of shelled oil palm kernels caused by seedborne fungi , 1987 .

[2]  B. Oso Thermophilic fungi and the deterioration of nigerian oil palm kernels , 2008, Economic Botany.

[3]  Mohd Ali Hassan,et al.  Thermophilic biohydrogen production from palm oil mill effluent (POME) using suspended mixed culture. , 2010 .

[4]  Y. Tur’yan,et al.  Acid Value Determination in Vegetable Oils by Indirect Titration in Aqueous-alcohol Media , 2005 .

[5]  P. Schloss,et al.  Tracking temporal changes of bacterial community fingerprints during the initial stages of composting. , 2003, FEMS microbiology ecology.

[6]  Poonsuk Prasertsan,et al.  Optimization of simultaneous thermophilic fermentative hydrogen production and COD reduction from palm oil mill effluent by Thermoanaerobacterium-rich sludge , 2008 .

[7]  M. Dickinson,et al.  Use of terminal restriction fragment length polymorphism (T‐RFLP) for identification of phytoplasmas in plants , 2006 .

[8]  S. Odunfa Bacteria involved in the deterioration of Nigerian palm oil under storage , 1989 .

[9]  P. Bechtel,et al.  Salmon by-product storage and oil extraction , 2008 .

[10]  Elaine E. Vaughan,et al.  Diversity, Dynamics, and Activity of Bacterial Communities during Production of an Artisanal Sicilian Cheese as Evaluated by 16S rRNA Analysis , 2002, Applied and Environmental Microbiology.

[11]  Kwasi Poku,et al.  Small-scale palm oil processing in Africa. , 2002 .

[12]  J. Thioulouse,et al.  Characterization of Bacterial and Fungal Soil Communities by Automated Ribosomal Intergenic Spacer Analysis Fingerprints: Biological and Methodological Variability , 2001, Applied and Environmental Microbiology.

[13]  M. C. Zamora,et al.  The correlation between water activity and % moisture in honey : fundamental aspects and application to Argentine honeys , 2006 .

[14]  B. Oso Thermophilic fungi from stacks of oil palm kernels in Nigeria. , 1974, Zeitschrift fur allgemeine Mikrobiologie.

[15]  S. Pérez-Luz,et al.  Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region , 2004, Journal of applied microbiology.

[16]  E. Paul,et al.  Terminal Restriction Fragment Length Polymorphism Data Analysis for Quantitative Comparison of Microbial Communities , 2003, Applied and Environmental Microbiology.

[17]  Danilo Ercolini,et al.  Bacterial Community Structure and Location in Stilton Cheese , 2003, Applied and Environmental Microbiology.

[18]  John William Purseglove,et al.  Tropical crops: monocotyledons , 1972 .

[19]  L. Ingram,et al.  Cloning and Characterization of the Zymobacter palmae Pyruvate Decarboxylase Gene (pdc) and Comparison to Bacterial Homologues , 2002, Applied and Environmental Microbiology.

[20]  G. L. French,et al.  Rapid Diagnosis of Bacteremia by Universal Amplification of 23S Ribosomal DNA Followed by Hybridization to an Oligonucleotide Array , 2000, Journal of Clinical Microbiology.

[21]  J. Clarke,et al.  Lipolytic activity of fungi on rapeseed oil , 1981 .

[22]  G. Zervakis,et al.  Edible mushrooms from olive oil mill wastes , 1996 .