Role of fluorescent pseudomonas associated with root nodules of mungbean in the induction of nodulation by the rhizobia in mungbean.

Fluorescent Pseudomonas has been reported to associate with root nodules of a number of plants and play role in plant disease suppression and increased plant growth. However their role in nodule formation is still unclear. In a gnotobiotic experiment, combined effect of two isolates each of fluorescent Pseudomonas (NAFP-19 and NAFP-32) and rhizobia (Rhizobium vignae (NFB-103) and R. vignae (NFB-109) on nodules of mungbean was evaluated. The results showed an increase in the number of nodule primordia, mature nodules and the concentration of nitrogen and phosphorus in mungbean plants. In screen house experiment, garden soil which was naturally infested with root rotting fungi and rhizobia was used. Dual application of Pseudomonas and rhizobia in the garden soil produced greater number of nodules per plant and higher nitrogen fixation than when rhizobia were used alone. Dual application of bacteria also suppressed root rotting fungi. The results showed that nodule associated fluorescent Pseudomonas played a role in plant-rhizobia symbiosis.

[1]  P. Martínez-Hidalgo,et al.  The Nodule Microbiome: N2-Fixing Rhizobia Do Not Live Alone , 2017 .

[2]  Habiba,et al.  ROLE OF MUNGBEAN ROOT NODULE ASSOCIATED FLUORESCENT PSEUDOMONAS AND RHIZOBIA IN SUPPRESSING THE ROOT ROTTING FUNGI AND ROOT KNOT NEMATODES IN CHICKPEA ( C ICER ARIETINUM L . ) , 2016 .

[3]  Habiba,et al.  EVALUATION OF BIOCONTROL POTENTIAL OF EPIPHYTIC FLUORESCENT PSEUDOMONAS ASSOCIATED WITH HEALTHY FRUITS AND VEGETABLES AGAINST ROOT ROT AND ROOT KNOT PATHOGENS OF MUNGBEAN , 2016 .

[4]  S. Ehteshamul-Haque,et al.  INDUCTION OF SYSTEMIC RESISTANCE IN COTTON BY THE NEEM CAKE AND PSEUDOMONAS AERUGINOSA UNDER SALINITY STRESS AND MACROPHOMINA PHASEOLINA INFECTION , 2016 .

[5]  S. Ehteshamul-Haque,et al.  Evaluation of biocontrol potential of fluorescent Pseudomonas associated with root nodules of mungbean , 2015 .

[6]  S. Ehteshamul-Haque,et al.  EFFECT OF ENDOPHYTIC PSEUDOMONAS AERUGINOSA AND TRICHODERMA HARZIANUM ON SOIL-BORNE DISEASES , MYCORRHIZAE AND INDUCTION OF SYSTEMIC RESISTANCE IN OKRA GROWN IN SOIL AMENDED WITH VERNONIA ANTHELMINTICA ( L . ) SEED ’ S POWDER , 2015 .

[7]  S. Ehteshamul-Haque,et al.  MANAGEMENT OF ROOT ROT AND ROOT KNOT DISEASE OF MUNGBEAN WITH THE APPLICATION OF MYCORRHIZOSPHERIC FLUORESCENT PSEUDOMONAS UNDER FIELD CONDITION , 2014 .

[8]  S. Ehteshamul-Haque,et al.  MANAGING THE ROOT DISEASES OF OKRA WITH ENDO-ROOT PLANT GROWTH PROMOTING PSEUDOMONAS AND TRICHODERMA VIRIDE ASSOCIATED WITH HEALTHY OKRA ROOTS , 2013 .

[9]  M. Khan,et al.  Effect of Plant Growth-Promoting Rhizobacteria on Growth, Nodulation and Nutrient Accumulation of Lentil Under Controlled Conditions , 2012 .

[10]  Varun Kumar,et al.  Comparison of nodC and 16S rDNA gene analysis of rhizobia associated with legumes of arid and semi-arid regions of Haryana , 2012 .

[11]  D. Choudhary,et al.  Molecular Characterization of Pseudomonas spp. Isolated from Root Nodules of Various Leguminous Plants of Shekhawati Region, Rajasthan, India , 2012 .

[12]  Ana Brândusa Pavel,et al.  PyElph - a software tool for gel images analysis and phylogenetics , 2012, BMC Bioinformatics.

[13]  G. O’Hara,et al.  Enhanced nodulation and symbiotic effectiveness of Medicago truncatula when co-inoculated with Pseudomonas fluorescens WSM3457 and Ensifer (Sinorhizobium) medicae WSM419 , 2011, Plant and Soil.

[14]  M. El-sheekh,et al.  Effect of two species of cyanobacteria as biofertilizers on some metabolic activities, growth, and yield of pea plant , 2010, Biology and Fertility of Soils.

[15]  G. Berg,et al.  Co-inoculation of Pseudomonas spp. with Rhizobium improves growth and symbiotic performance of fodder galega (Galega orientalis Lam.) , 2010 .

[16]  C. S. Piper.  Soil and Plant Analysis: A Laboratory Manual of Methods for the Examination of Soils and the Determination of the Inorganic Constituents of Plants , 2010 .

[17]  D. Real,et al.  Prospects For The Future Use Of Legumes , 2008 .

[18]  D. Egamberdieva.  Plant Growth Promoting Properties of Rhizobacteria Isolated from Wheat and Pea Grown in Loamy Sand Soil , 2008 .

[19]  R. Dubey,et al.  Rhizosphere competent Mesorhizobiumloti MP6 induces root hair curling, inhibits Sclerotinia sclerotiorum and enhances growth of Indian mustard (Brassica campestris) , 2007 .

[20]  S. Vesper,et al.  Enhanced nodulation of soybean by Bradyrhizobium in the presence ofPseudomonas fluorescens , 1988, Plant and Soil.

[21]  E. Watkin,et al.  Medicago sativa and Medicago murex differ in the nodulation response to soil acidity , 2002, Plant and Soil.

[22]  S. Ehteshamul-Haque,et al.  Suppression of the root rot–root knot disease complex by Pseudomonas aeruginosa in tomato: The influence of inoculum density, nematode populations, moisture and other plant-associated bacteria , 2001, Plant and Soil.

[23]  A. Ghaffar,et al.  Biological control of root rot-root knot disease complex of tomato , 2000, Plant and Soil.

[24]  Y. Zafar,et al.  Rhizobium, Bradyrhizobium and Agrobacterium strains isolated from cultivated legumes , 2004, Biology and Fertility of Soils.

[25]  W. Małek,et al.  Variation in symbiotic characters of alfalfa cultivars inoculated with Sinorhizobium meliloti strains , 2001, Biology and Fertility of Soils.

[26]  H. Spaink.  Root nodulation and infection factors produced by rhizobial bacteria. , 2000, Annual review of microbiology.

[27]  D. J. Petersen,et al.  Influence of indoleacetic-acid-producing Bacillus isolates on the nodulation of Phaseolus vulgaris by Rhizobium etli under gnotobiotic conditions , 1996 .

[28]  A. Ghaffar,et al.  Use of Rhizobia in the Control of Root Rot Diseases of Sunflower, Okra, Soybean and Mungbean , 1993 .

[29]  S. Pueppke.  Physiology of nodule initiation in the nitrogen‐fixing legume‐Rhizobium symbiosis , 1986 .

[30]  K. A. Gomez,et al.  Statistical Procedures for Agricultural Research. , 1984 .

[31]  J. Kloepper,et al.  Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria , 1980, Nature.

[32]  A. Ghaffar,et al.  Population study of the sclerotia of Macrophomina phaseolina in cotton fields , 1975 .

[33]  W. Snyder,et al.  Quantitative estimations by plate counts of propagules of the bean root rot Fusarium in field soils , 1962 .

[34]  S. Wilhelm.  Longevity of the Verticillium wilt fungus in the laboratory and field , 1955 .