MONOCLONAL ANTIBODY‐RICIN CONJUGATE CYTOTOXIC TO CELLS EXPRESSING THE COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN (CALLA)

&NA; The monoclonal antibody PHM‐6, which is specific for the common acute lymphoblastic leukemia antigen (CALLA), was conjugated to the plant toxin ricin. Binding of the PHM‐6‐ricin conjugate to cells via the ricin molecule was blocked by the presence of 100 mM lactose. The IC50 (concentration resulting in 50% inhibition) of the PHM‐6‐ricin conjugate for the CALLA‐positive KM‐3 cell line was 280‐fold greater than for bone marrow stem cells, indicating the potential of this conjugate for immunological purging of autologous remission marrow.

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