Methods of isolation, purification and quantitation of bovine immunoglobulins: a technical review.

Summary The Mancini method for the measurement of bovine immunoglobulin classes, and the preparation of pure antigens and antibodies are described in thorough technical detail. Zusammenfassung Methoden der Isolierung, Reinigung und quantitativen Bestimmung von Rinder-Immunglobulinen Ein uberblick uber die Technik Es wird eine grundliche Beschreibung von technischen Details der Mancini-Methode zum Nachweis der Rinder-Immunglobulinklassen gegeben. Ferner werden die Methoden zur Praparation reiner Antigene und Antikoorper geschildert. Resume Methodes d'isolement, de purification et de determination quantitative des immunoglobulines bovines. Un apercu sur la technique Les details techniques de la methode de Mancini pour la mise en evidence des classes d'immunoglobulines bovines sont decrits de facon approfondie. On presente les methodes de preparation des antigenes et des anticorps purs. Resumen Metodos de aislamiento, purificacion valoracion y cuantitativa de las inmunoglobulinas bovinas. Una vision de conjunto sobre las tecnicas Se facilita una descripcion exhaustiva de detalles tecnicos relativos al procedimiento de Mancini para identificar las clases de inmunoglobulinas vacunas. Tambien se describen los metodos para preparar antigenos y anticuerpos puros.

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