In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms, E. faecalis, C. albicans, and S. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity against E. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity against C. albicans and S. aureus compared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity against E. faecalis, C. albicans, and S. aureus.

[1]  M. Inci,et al.  Antifungal activity of several root canal sealers against Candida albicans , 2013, Acta odontologica Scandinavica.

[2]  S. Durmaz,et al.  In vitro antimicrobial efficiency of different root canal sealers against Enterecoccus faecalis , 2013 .

[3]  E. Kontakiotis,et al.  Comparative in vitro antibacterial activity of six root canal sealers against Enterococcus faecalis and Proteus vulgaris. , 2012, Journal of investigative and clinical dentistry.

[4]  C. Koga‐Ito,et al.  In vitro antimicrobial activity of AH Plus, EndoREZ and Epiphany against microorganisms. , 2012, Indian journal of dental research : official publication of Indian Society for Dental Research.

[5]  C. Sedgley,et al.  Antibacterial activity of endosequence root repair material and proroot MTA against clinical isolates of Enterococcus faecalis. , 2011, Journal of endodontics.

[6]  F. Tay,et al.  Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. , 2011, Journal of endodontics.

[7]  S. Arabia.  EVALUATION OF ANTIMICROBIAL EFFECT OF ROOT CANAL SEALERS , 2011 .

[8]  Ya Shen,et al.  Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. , 2009, Journal of endodontics.

[9]  B. Peng,et al.  Assessment of a new root canal sealer's apical sealing ability. , 2009, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[10]  Takashi Saito,et al.  In vitro evaluation of the antimicrobial activity of a new resin-based endodontic sealer against endodontic pathogens. , 2008, Journal of oral science.

[11]  E. Weiss,et al.  Antibacterial properties of four endodontic sealers. , 2008, Journal of endodontics.

[12]  F. Tay,et al.  Water sorption and solubility of methacrylate resin-based root canal sealers. , 2007, Journal of endodontics.

[13]  Wanted: a base of evidence. , 2007, Journal of endodontics.

[14]  A. Erdemir,et al.  Assessment of antibacterial activity of EndoREZ. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[15]  S. Schwartz,et al.  nterococcus faecalis: Its Role in Root Canal Treatment ailure and Current Concepts in Retreatment , 2006 .

[16]  Aravind,et al.  Comparative evaluation of the antimicrobial efficacy of five endodontic root canal sealers against Enterococcus faecalis and Candida albicans , 2006 .

[17]  G. Pizzo,et al.  In vitro antibacterial activity of endodontic sealers. , 2006, Journal of dentistry.

[18]  C. R. Sipert,et al.  In vitro antimicrobial activity of Fill Canal, Sealapex, Mineral Trioxide Aggregate, Portland cement and EndoRez. , 2005, International endodontic journal.

[19]  M. Haapasalo,et al.  Clinical aspects related to endodontic yeast infections , 2004 .

[20]  Brenda Paula Figueiredo de Almeida Gomes,et al.  In vitro evaluation of the antimicrobial activity of five root canal sealers. , 2003, Brazilian dental journal.

[21]  M. Haapasalo,et al.  Enterococcus faecalis– the root canal survivor and ‘star’ in post-treatment disease , 2003 .

[22]  M. T. Filho,et al.  Release of formaldehyde by 4 endodontic sealers. , 1999, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[23]  Andrea Edith Kaplan,et al.  Antimicrobial effect of six endodontic sealers: an in vitro evaluation. , 1999, Endodontics & dental traumatology.

[24]  Z. Fuss,et al.  Assessment of antibacterial activity of endodontic sealers by a direct contact test. , 1996, Endodontics & dental traumatology.

[25]  M. T. Jiménez de Anta,et al.  Antimicrobial activity of seven root canal sealers. Results of agar diffusion and agar dilution tests. , 1992, Oral surgery, oral medicine, and oral pathology.